The nuclear pore complex (NPC) is the sole passageway for the transport of macromolecules across the nuclear envelope. Nup133, a major component in the essential Y-shaped Nup84 complex, is a large scaffold protein of the NPC's outer ring structure. Here, we describe an integrative modeling approach that produces atomic models for multiple states of Saccharomyces cerevisiae (Sc) Nup133, based on the crystal structures of the sequence segments and their homologs, including the related Vanderwaltozyma polyspora (Vp) Nup133 residues 55 to 502 (VpNup133(55-502)) determined in this study, small angle X-ray scattering profiles for 18 constructs of ScNup133 and one construct of VpNup133, and 23 negative-stain electron microscopy class averages of ScNup133(2-1157). Using our integrative approach, we then computed a multi-state structural model of the full-length ScNup133 and validated it with mutational studies and 45 chemical cross-links determined via mass spectrometry. Finally, the model of ScNup133 allowed us to annotate a potential ArfGAP1 lipid packing sensor (ALPS) motif in Sc and VpNup133 and discuss its potential significance in the context of the whole NPC; we suggest that ALPS motifs are scattered throughout the NPC's scaffold in all eukaryotes and play a major role in the assembly and membrane anchoring of the NPC in the nuclear envelope. Our results are consistent with a common evolutionary origin of Nup133 with membrane coating complexes (the protocoatomer hypothesis); the presence of the ALPS motifs in coatomer-like nucleoporins suggests an ancestral mechanism for membrane recognition present in early membrane coating complexes.

The top quark and electroweak bosons (W and Z) represent the most massive fundamental particles yet discovered, and as such refer directly to the Standard Model's greatest remaining mystery: the mechanism by which all particles gained mass. This report summarizes the work done within the top-ew group of the Tevatron-for-LHC workshop. It represents a collection of both Tevatron results, and LHC predictions. The hope is that by considering and comparing both machines, the LHC program can be improved and aided by knowledge from the Tevatron, and that particle physics as a whole can be enriched. The report includes measurements of the top quark mass, searches for single top quark production, and physics of the electroweak bosons at hadron colliders.

Measurements are presented of the differential cross sections for Z bosons produced in proton-proton collisions at s = 13 TeV and decaying to muons and electrons. The data analyzed were collected in 2016 with the CMS detector at the LHC and correspond to an integrated luminosity of 35.9 fb−1. The measured fiducial inclusive product of cross section and branching fraction agrees with next-to-next-to-leading order quantum chromodynamics calculations. Differential cross sections of the transverse momentum pT, the optimized angular variable ϕη∗, and the rapidity of lepton pairs are measured. The data are corrected for detector effects and compared to theoretical predictions using fixed order, resummed, and parton shower calculations. The uncertainties of the measured normalized cross sections are smaller than 0.5% for ϕη∗< 0.5 and for pTZ< 50 GeV. [Figure not available: see fulltext.]