A genomic view of the human-Bacteroides thetaiotaomicron symbiosis

Science. 2003 Mar 28;299(5615):2074-6. doi: 10.1126/science.1080029.

Abstract

The human gut is colonized with a vast community of indigenous microorganisms that help shape our biology. Here, we present the complete genome sequence of the Gram-negative anaerobe Bacteroides thetaiotaomicron, a dominant member of our normal distal intestinal microbiota. Its 4779-member proteome includes an elaborate apparatus for acquiring and hydrolyzing otherwise indigestible dietary polysaccharides and an associated environment-sensing system consisting of a large repertoire of extracytoplasmic function sigma factors and one- and two-component signal transduction systems. These and other expanded paralogous groups shed light on the molecular mechanisms underlying symbiotic host-bacterial relationships in our intestine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology
  • Bacteroides / genetics*
  • Bacteroides / physiology
  • Biological Evolution
  • Carbohydrate Metabolism
  • Chromosomes, Bacterial / genetics
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Genome, Bacterial*
  • Humans
  • Interspersed Repetitive Sequences
  • Intestines / microbiology*
  • Physical Chromosome Mapping
  • Polysaccharides / metabolism
  • Proteome
  • Sequence Analysis, DNA*
  • Sigma Factor / genetics
  • Sigma Factor / physiology
  • Signal Transduction
  • Symbiosis*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Polysaccharides
  • Proteome
  • Sigma Factor