On-line monitoring of hybridoma cell growth using a laser turbidity sensor

Biotechnol Bioeng. 1992 Dec 20;40(11):1337-42. doi: 10.1002/bit.260401107.

Abstract

A high-sensitivity turbidity probe was used for on-line monitoring of the cell concentration in batch hybridoma cultivation. Good correlation between off-line cell counts and the linearized sensor signal was found. The quality of the signal was sufficiently high to provide for on-line estimation of the specific growth rate using an efficient filtering procedure. These positive results suggest that such laser turbidity sensors will facilitate development of systems for on-line monitoring and control of animal cell cultivations.