Antioxidant and immunomodulatory activity of selenium exopolysaccharide produced by Lactococcus lactis subsp. lactis

Food Chem. 2013 May 1;138(1):84-9. doi: 10.1016/j.foodchem.2012.10.029. Epub 2012 Nov 2.

Abstract

Exopolysaccharide (EPS) was isolated and purified from Lactococcus lactis subsp. Lactis culture broth. Selenium chloride oxide (SeCl(2)O) was added to the EPS to synthesize selenium-exopolysaccharide (Se-EPS). The in vitro and in vivo antioxidant and in vivo immunomodulatory activity of EPS and Se-EPS were compared. EPS and Se-EPS scavenged superoxide anions and hydroxyl radicals. They also increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, while decreasing malondialdehyde (MDA) levels in serum and in the livers of mice. Se-EPS showed stronger in vitro and in vivo antioxidant activity than were shown by EPS. The in vivo immunoenhancement activity of EPS and Se-EPS induced by cyclophosphamide (CY) treatment in immunosuppressed mice was researched. EPS and Se-EPS treatments increased macrophage phagocytosis, spleen and thymus indices and haemolytic complement activity (HC(50)). Se-EPS showed stronger immunomodulatory activity than did EPS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / metabolism
  • Antioxidants / pharmacology*
  • Female
  • Immunologic Factors / chemistry
  • Immunologic Factors / metabolism
  • Immunologic Factors / pharmacology*
  • Lactococcus lactis / chemistry*
  • Lactococcus lactis / metabolism
  • Macrophages / drug effects
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Phagocytosis / drug effects
  • Polysaccharides, Bacterial / chemistry
  • Polysaccharides, Bacterial / metabolism
  • Polysaccharides, Bacterial / pharmacology*
  • Selenium / metabolism*
  • Selenium / pharmacology

Substances

  • Antioxidants
  • Immunologic Factors
  • Polysaccharides, Bacterial
  • Selenium