Dietary fermentable fiber upregulated immune related genes expression, increased innate immune response and resistance of rainbow trout (Oncorhynchus mykiss) against Aeromonas hydrophila

Fish Shellfish Immunol. 2014 Dec;41(2):326-31. doi: 10.1016/j.fsi.2014.09.007. Epub 2014 Sep 10.

Abstract

This trial was carried out to investigate the effects of dietary administration of Vitacel(®), a commercial fermentable fiber, on immune related genes (Lysozyme, TNFα and HSP70) expression, innate immune response and resistance of rainbow trout against Aeromonas hydrophila. 120 healthy rainbow trout (81.65 ± 1.49 g) were distributed in six fiberglass tanks assigned to two treatments. The treatments were feeding rainbow trout with diets supplemented with 0 (control) or 10 g kg(-1) Vitacel(®) for 45 days. The results revealed that administration of fermentable fiber significantly (P < 0.05) upregulated lysozyme and TNFα gene expression. HSP70 gene expression was significantly lower in Vitacel(®) fed fish at the end of trial (P < 0.05). Furthermore dietary administrations of Vitacel(®) remarkably elevated rainbow trout innate immune parameters include serum lysozyme, ACH50, bactericidal activity and agglutination antibody titer (P < 0.05). Administration of 10 g kg(-1) Vitacel(®) significantly increased rainbow trout resistance against A. hydrophila (P < 0.05). The results of present study revealed that dietary Vitacel(®) can upregulates immune related genes expression and elevates innate immune response and disease resistance of rainbow trout.

Keywords: Disease resistance; Fermentable fiber; Immune genes expression; Immune response; Rainbow trout.

MeSH terms

  • Aeromonas hydrophila / immunology*
  • Agglutination Tests / veterinary
  • Animals
  • DNA Primers / genetics
  • Dietary Fiber / pharmacology*
  • Disease Resistance / drug effects
  • Disease Resistance / immunology*
  • Fermentation
  • Fish Diseases / immunology
  • Fish Diseases / microbiology*
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / veterinary*
  • HSP70 Heat-Shock Proteins / metabolism
  • Immunity, Innate / drug effects*
  • Muramidase / metabolism
  • Oncorhynchus mykiss*
  • Real-Time Polymerase Chain Reaction / veterinary
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • DNA Primers
  • Dietary Fiber
  • HSP70 Heat-Shock Proteins
  • Tumor Necrosis Factor-alpha
  • Muramidase