Characteristics and immune-enhancing activity of pectic polysaccharides from sweet cherry (Prunus avium)

Food Chem. 2018 Jul 15:254:47-54. doi: 10.1016/j.foodchem.2018.01.145. Epub 2018 Jan 31.

Abstract

Two water soluble polysaccharides components PAPS-1 and PAPS-2 with homogeneously distributed molecular weight were obtained from Prunus avium. PAPS-1 and PAPS-2 contained GalA: Ara: Gal: Rha: GluA: Glu in 49.38: 32.39: 10.68: 4.66: 1.94: 0.48 and 77.18: 14.91: 3.39: 3.46: 0.93: 0.19 M ratios respectively, as well as trace amount of mannose and fucose. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR) and methylation analysis indicated that both fractions were type I rhamnogalacturonan (RG-I) pectic polysaccharides with glycan side chains constituted mainly of arabinose with minor amount of galactose. Galacturonic acid methylation and sugar acetylation was found in both PAPS-1 and PAPS-2. Both PAPS-1 and PAPS-2 significantly induced the NO release from RAW264.7 cells and the expression of several immune-related molecular (TNFα, IL6, IL10, GCSF, iNOS, COX-2) was induced in RAW264.7 cells.

Keywords: Characteristics; Immune-enhancing activity; Polysaccharides; Prunus avium.

MeSH terms

  • Animals
  • Galactose / analysis
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Hexuronic Acids / analysis
  • Immunologic Factors / chemistry*
  • Immunologic Factors / pharmacology*
  • Mice
  • Molecular Weight
  • Pectins / chemistry*
  • Pectins / pharmacology*
  • Prunus avium / chemistry*
  • RAW 264.7 Cells

Substances

  • Hexuronic Acids
  • Immunologic Factors
  • galacturonic acid
  • Pectins
  • Galactose