Abstract
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldose-Ketose Isomerases*
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Base Sequence
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Carbohydrate Epimerases / genetics*
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Carbohydrate Epimerases / metabolism
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Ethanol / metabolism*
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Fermentation*
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Genes, Bacterial*
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Molecular Sequence Data
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Phosphoglycerate Kinase / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Thermus thermophilus / genetics*
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Xylose / metabolism*
Substances
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Recombinant Proteins
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Ethanol
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Xylose
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Phosphoglycerate Kinase
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Carbohydrate Epimerases
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Aldose-Ketose Isomerases
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xylose isomerase
Associated data
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GENBANK/A10243
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GENBANK/D90256
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GENBANK/J05650
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GENBANK/L09699
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GENBANK/M15050
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GENBANK/M36269
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GENBANK/M57384
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GENBANK/M73789
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GENBANK/M84564
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GENBANK/M91248
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GENBANK/S73809
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GENBANK/X00772
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GENBANK/X02795
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GENBANK/X16042
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GENBANK/X57599
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GENBANK/X59466
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GENBANK/X61059