Exercise Mitigates Endothelial Pyroptosis and Atherosclerosis by Downregulating NEAT1 Through N6-Methyladenosine Modifications

Qingyuan Yang; Shiliang Chen; Xingyi Wang; Xinyu Yang; Liangqi Chen; Tuo Huang; Yang Zheng; Xianghui Zheng; Xiaojun Wu; Yong Sun; Jian Wu

doi:10.1161/ATVBAHA.123.319251

Exercise Mitigates Endothelial Pyroptosis and Atherosclerosis by Downregulating NEAT1 Through N6-Methyladenosine Modifications

Arterioscler Thromb Vasc Biol. 2023 Jun;43(6):910-926. doi: 10.1161/ATVBAHA.123.319251. Epub 2023 Apr 20.

Authors

Qingyuan Yang^{1

2

3}, Shiliang Chen^{1

2

3}, Xingyi Wang^{1

2

3}, Xinyu Yang^{1

2

3}, Liangqi Chen^{1

2

3}, Tuo Huang^{1

2

3}, Yang Zheng^{1

2

3}, Xianghui Zheng^{1

2

3}, Xiaojun Wu^{1

2

3}, Yong Sun^{2

3}, Jian Wu^{1

2

3}

Affiliations

¹ Department of Cardiology, Cardiac Rehabilitation Center (Q.Y., S.C., X. Wang, X.Y., L.C., T.H., Y.Z., X.Z., X. Wu, J.W.), Second Affiliated Hospital of Harbin Medical University, Heilongjiang, China.
² Department of Cardiology (Q.Y., S.C., X. Wang, X.Y., L.C., T.H., Y.Z., X.Z., X. Wu, Y.S., J.W.), Second Affiliated Hospital of Harbin Medical University, Heilongjiang, China.
³ The Key Laboratory of Myocardial Ischaemia, Harbin Medical University, Ministry of Education, Heilongjiang, China (Q.Y., S.C., X. Wang, X.Y., L.C., T.H., Y.Z., X.Z., X. Wu, Y.S., J.W.).

PMID: 37078289
DOI: 10.1161/ATVBAHA.123.319251

Abstract

Background: The benefits of exercise on the cardiovascular system are widely recognized; however, the underlying mechanisms are unknown. Here, we report the effect of the long noncoding RNA NEAT1 (nuclear paraspeckle assembly transcript 1), which is regulated by exercise, on atherosclerosis development after N6-methyladenosine (m6A) modifications.

Methods: Using clinical cohorts and NEAT1^-/- mice, we determined the exercise-mediated expression and role of NEAT1 in atherosclerosis. To investigate the mechanism of epigenetic modification of NEAT1 regulated by exercise, we identified METTL14 (methyltransferase-like 14)-a key m6A modification enzyme under exercise-and found that METTL14 alters the expression and role of NEAT1 through m6A modification and elucidated the specific mechanism of METTL14 in vitro and in vivo. Finally, the NEAT1 downstream regulatory network was investigated.

Results: We found that NEAT1 expression was downregulated with exercise and that downregulation of NEAT1 was an important factor in the improvement of atherosclerosis with exercise. Exercise-mediated loss of function of NEAT1 can delay atherosclerosis. Mechanistically, we showed that exercise induced a significant downregulation of m6A modification and METTL14, which binds to the m6A sites of NEAT1 and promotes NEAT1 expression through subsequent YTHDC1 (YTH domain-containing 1) recognition to promote endothelial pyroptosis. Furthermore, NEAT1 induces endothelial pyroptosis by binding KLF4 (Kruppel-like factor 4) to promote the transcriptional activation of the key pyroptotic protein NLRP3 (NOD-like receptor thermal protein domain-associated protein 3), whereas exercise can attenuate NEAT1-mediated endothelial pyroptosis to improve atherosclerosis.

Conclusions: Our study of NEAT1 provides new insights into the improvement of atherosclerosis by exercise. This finding demonstrates the role of exercise-mediated NEAT1 downregulation in atherosclerosis while expanding our understanding of the mechanisms by which exercise regulates long noncoding RNA function through epigenetic modifications.

Keywords: atherosclerosis; downregulation; endothelial cells; exercise; mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine
Animals
Atherosclerosis* / genetics
Atherosclerosis* / prevention & control
Mice
Pyroptosis
RNA, Long Noncoding* / genetics

Substances

Adenosine
RNA, Long Noncoding
NEAT1 long non-coding RNA, mouse