Biology

Biology - American National Curriculum questions asked by Filo students

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Question 1

Which of the following best describes the genetic complement in each daughter cell at the conclusion of meiosis?A. A diploid set of chromosomes identical to the other daughter cells' chromosomesB. A diploid set of chromosomes that are identical to the parents' chromosomesC. A haploid set of chromosomes that are a recombination of the parents' chromosomesD. A haploid set of chromosomes that are identical to the parents' chromosomes

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Question 2

Title: Purification and Identification of Recombinant Plasmid DNA pAKThe identification of plasmids is based on its selection marker’s function and its molecular structure. In our lab work so far, the intended goal was to prepare a recombinant plasmid with two selection markers: ampR & kanR genes. This was achieved through the HindIII/BamHI double digestion followed by ligation and transformation. The transformation result by functional screening has been obtained by you. From these results the ligation plates (Lig/LB/amp and Lig/LB/kan) have been used to verify the recombinant from non-recombinant plasmids by implementing the replica analysis. You are asked to:B. You should suggest the use of polymerase chain reaction, DNA sequencing and bioinformatics tools to verify the recombinant nature of the isolated plasmid.Your answer should cover the following:· Goal: · Objectives: Hypothesis: Methodology: · Use the methods from lab 1 until 6 (for preparation of the recombinant plasmid) and show the results.· Use labs 7 until 8 and PCR chapter to the recombinant nature of the isolated plasmid and the expected results. · Materials & Methods: Part A · Expected results Part B

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Question 3

Insertion sequence contains inverted repeats_ When two plasmids containing such inverted repeats are aligned closed together; of the two plasmids will results in of the two plasmids_A. homologous recombination, cointegrationB. homologous recombination, transformationC. conjugation, transformationD. conjugation, cointegration

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Question 4

2 Meiosis and Sexual Reproduction (conthwed) CHECK YOUR PROGRESS 1. Explain how the structure of DNA determines the structure of proteins which carry out mast of the work of cells. Pea Contains sequence

2. Assess how meiosis contributes to genetic variation, while mitosis does not

3. Illustrate how nondisjunction occurs during meiosis.

4. Summarize the role differentiation plays in the production and maintenance of a complex organism.

5. Describe a possible application for stem cells.

6. Compare and contrast mitosis and meiosis by creating a Venn diagram.

7. Conduct research on the consequences of nondisjunction other than trisomy 21. Write a paragraph about your findings.

Science Notebook - Cellular Reproduction and Sexual Reproduction 124

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Question 5

STUDENT HANDOUT Guiding Questions for Historici. Name

Complete the following chart with your group after studies presented by other groups in your notebook, verioo CASE STUDY: 7. What core values were in conflict in this case? Q Northwest Association for Biomedical Research HUMANS IN RESEARCH

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Question 6

Text: Come into lab and water leak has washed off all of the labels from your vials. Because it is not possible to distinguish these fly genotypes by looking at them, you decide to extract genomic DNA and perform a PCR reaction to determine which vial contains your homozygous aa flies. You know that the allele is the result of a 400 bp deletion. You design PCR primers that hybridize to this locus upstream and downstream of the gene, and run a PCR reaction and gel (shown below). Which vial contains the homozygous recessive flies? Lane A Lane B Lane C It is not possible to tell Both A and C

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Question 7

Please help. Question 31 1 pt Shown below is a diagram for a plasmid vector you want to use to clone a gene. The diagram shows the location of the recognition sites for four restriction enzymes, BamHI (B), EcoRI (E), HindIII (H), and XhoI (X). The genes encoding beta-lactamase (AmpR) and beta-galactosidase (lacZ) are indicated. If you were to use this vector, which enzyme should be used to linearize the plasmid in preparation for cloning? B acz Amp OHindIII OBamHI O EcoRI OXhoI EcoRI and XhoI

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Question 8

3. A cladogram based on physical morphologies was created to show the evolutionary relationships between several species. Later, DNA sequences were obtained for the same gene in these organisms. Those sequences are shown below. Evaluate the sequencing data to determine whether it supports the existing cladogram. If there are any inaccuracies, explain how they can be corrected. (7 points) Original cladogram: Camel Hippo Whale Organism DNA Sequence 5'... AATCGCGATATACGCGTATACGTGTCAGTCTGTACGTAGCCTTAGC...3' Camel 5'... AATCGCGATATACGCGTATACGTGTCAGTCTGTACGTAGAGTTAAC...3' Whale 5'... AATCGCGATATACGCGTATACGTGTCAGTCTGTACGTAGAGTTACC...3' Hippo 5'... AATCGCGATATACGCGTATACGTGTCAGTCTGTACGTAGATTTAGC...3' Pig

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Question 9

punnett square practice 1. Let's say that in seals, the gene for the length of the whiskers has two alleles. The dominant allele

(W)

codes long whiskers and the recessive allele (

\frac{w ) codes}{5/ d}

for short whiskers. Lory a. What is the probability of producing offspring that have short whiskers from a cross of two long-whiskered seals, one that is homozygous dominant and one that is heterozygous? Show your work on the punnett square.

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Question 10

punnett square practioe 1. Lets say that in seals, the gene for the length of the whiskers has two alleles. The dominant allele (W) codes long whiskers and the recessive allele (

W

) codes for short whiskers. Lony a. What is the probability of producing offspring that have short whiskers from a cross of two long-whiskered seals, one that is homozygous dominant and one that is heterezygeus? Show your work on the punnett square.

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Question 11

Examine the energy pyramid below. Tertiary Consumers (Ex. Coyotes) Secondary Consumers (Ex. Snakes and Hawks) Primary Consumers (Ex. Mice, Gophers, and Birds) Producers (Ex. Grasses, Tree Leaves, Flowers, and Vines) Is it possible for the top level to be wider than the level before it?A. Yes, if climatic conditions are right, there can be more predators than prey.B. No, it is not possible for a large population of coyotes to exist because of their mating habits.C. Yes, if the population of coyotes swells, the top level will be larger than the level before it.D. No, it is not possible for an ecosystem to support more predators than prey.

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Question 12

A short base sequence of mRNA and a table of the genetic code are shown below: Sequence of mRNA: AUGAGCCGAAGGUAGCUG Table of the genetic code: 2nd letter | 3rd letter | Amino Acid -----------|------------|----------- Phe | Leu | Leu Ser | Cys | Ser Cys | Ser | STOP STOP | Ser | STOP Pro | N? | 2 Ser | H | Ser MaU | START | 8 Outline the function of codons: 3b. Determine the sequence of amino acids that could be translated from the sequence of mRNA. Determine the DNA base sequence transcribed to form the sequence of mRNA.

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Question 13

'The genetic code has four codons for the amino acid Valine (GUG GUC, GUU, GUA) Organisms have evolved to use some codes more than other codes_ Which of the valine codes would you expect to be preferentially used in the genome of an algae growing in a hot spring at Yellowstone Park? EXPLAIN your answer'

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Question 14

given a protein sequence, can you think of additional functional annotations that were not covered by the case study? Can you find tracks in UCSC genome browser that are helpful for your annotation goals?

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Question 15

Incomplete Dominance 5. In humans straight hair (ss) and curly hair (cc) are incomplete dominant traits that result in hybrids who have wavy hair (sc). Cross a curly hair female with a wavy haired male. a. Complete a Punnett square for this cross. c. What are the chances of having a curly haired child?____________ Codominance and Multiple Allele 6. So far we have studied traits or genes that are coded for by just two alleles. However, some traits are coded for by more than two alleles. One of these is blood type in humans. In humans, there are four types of blood; type A, type B, type AB, and type O. The alleles A and B are codominant to each other and the O allele is recessive to both A and B alleles. So a person with the genotype AA or AO will have A type of blood. a. What possible genotypes will produce B type of blood? ________________________ b. What is the only genotype that will produce O type of blood? ____________________ c. What is the only genotype that will produce AB type of blood? ___________________ 7. Suppose a newborn baby was accidentally mixed up in the hospital. In an effort to determine the parents of the baby, the blood types of the baby and two sets of parents were determined. Baby 1 had type O Mrs. Brown had type B Mr. Brown had type AB Mrs. Smith had type B Mr. Smith had type B a. Draw Punnett squares for each couple (you may need to do more than 1 square/ couple) b. To which parents does baby #1 belong? Why?

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Question 16

Question \#3 What is the significance of the cycle between photosynthesis and cellular respiration for life on Earth?

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Question 17

You learn that MEW1 was 400nt and did not contain a restriction site for the ECORI restriction endonuclease, but MEW2 has a cut site 100bp from the 5'end and MEW 3 has a restriction site 200bp from the 5'end (and does not contain any other restriction sites). Please make a gel that has a size standard (ladder) in lane one and shows the genotypes of the following in the subsequent lanes (in order): I-1, I-2, III-2, IV-1, IV-4 What does your lane look like for individual I-1? select all of the bands that apply. MEW2 MEW2

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Question 18

Question 4 of 10 Individuals of a flowering plant species produce either blue or white flowers. Experimental crosses were carried out in controlled greenhouse conditions beginning with a parental generation of true-breeding plants (P1). An artificial pollination technique was used to make the crosses. The results from the two crosses are summarized in the table below: P1 Crosses: male blue female white male white female blue F1 progeny blue 450438 F1 progeny white When the F1 progeny were crossed with other F1 progeny, the following results were found: F1 Cross: male blue * female blue F2 progeny blue 360 F2 progeny white 120 Evaluate the data shown to determine whether they support the claim that heritable information passes from one generation to the next through meiosis followed by fertilization. Which of the following statements provides an accurate evaluation?A. A: The data are insufficient as shown and require results of a backcross between \mathrm{P} 1 and \mathrm{F} 1 to provide support for the claim.B. B: The data are insufficient as shown and require results of in vitro fertilization experiments to prove that meiosis is the mechanism of trait transfer.C. C: The two P1 results support the claim by demonstrating that the white trait did not show up in the F1 generation.D. D: The F2 results support the claim by demonstrating that the white trait was transferred via meiosis even though it remained hidden in the F1 generation.

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Question 19

Which of the following are consequences of global warming? Check All That Apply An alteration to global rainfall patterns An alteration to global wind patterns An increase in the amount of land covered by glaciers and polar caps An increase in political tensions between countries due to changes in agricultural patterns

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Question 20

What would have been a defensive disadvantage for Stegosaurus or other stegosaurs if their tail spikes had pointed upward (vertically) rather than outward (horizontally)?

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Biology - American National Curriculum questions asked by Filo students

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