Cellulose affinity chromatography adsorbent

Reverse transcriptase (from avian or murine RNA tumour viruses) [9068-38-6] [EC 2.7.7.49]. Purified by solubilising the virus with non-ionic detergent. Lysed virions were adsorbed on DEAE-cellulose or DEAE-Sephadex columns and the enzyme eluted with a salt gradient, then chromatographed on a phosphocellulose column and enzyme activity eluted in a salt gradient. Purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 7977 Smith Methods Enzymol 65 560 1980 see commercial catalogues for other transcriptases.]... [Pg.564]

Bulk cellulose is a poor affinity-chromatographic matrix its principal advantage is its low cost. This is rarely an overriding consideration since the enzymes to be purified are usually expensive. Cellulose was employed in 1953 in one of the earliest affinity-chromatography separations, where tyrosinase was purified on a column of aromatic cellulose ethers (27). Currently, cellulose (28) or related polysaccharides (29) are used in a number of applications as a bioselective adsorbent including DNA-cellulose for the purification of DNA-binding proteins. [Pg.1287]

An immobilized form of glycogen resulted when glycogen cyclic imidocarbonate reacted with a 6-aminohexyl derivative of agarose the immobilized glycogen derivative was used in the isolation of a-amylase by competitive affinity chromatography. Glycogen is adsorbed by magnetic iron oxide or cellulose coated with hydrous titanium(iv) oxide. ... [Pg.479]

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