Enzyme bound intermediates

The addition of COg to ribulose-l,5-bisphosphate results in the formation of an enzyme-bound intermediate, 2-carboxy,3-keto-arabinitol (Figure 22.24). This intermediate arises when COg adds to the enediol intermediate gener-... [Pg.731]

A transition to irreversible inhibitors is seen in pseudosubstrates forming enzyme-bound intermediates which are cleaved very slowly, for example, the 2-deoxy-2-fluoro-D-glycosides to be discussed in Section II,3,b. [Pg.349]

Endogenous NO is produced almost exclusively by L-arginine catabolism to L-citrul-line in a reaction catalyzed by a family of nitric oxide synthases (NOSs) [3]. In the first step, Arg is hydroxylated to an enzyme-bound intermediate "-hydroxy-1.-arginine (NHA), and 1 mol of NADPH (nicotinamide adenine dinucleotide phosphate, reduced form) and O2 are consumed. In the second step, N H A is oxidized to citrulline and NO, with consumption of 0.5 mol of NADPH and 1 mol of 02 (Scheme 1.1). Oxygen activation in both steps is carried out by the enzyme-bound heme, which derives electrons from NADPH. Mammalian NOS consists of an N-terminal oxy-... [Pg.4]

When an enzyme is mixed with a large excess of substrate (which is generally the case due to the high catalytic efficiency of enzymes), there is an initial period, the pre-steady state period, during which the concentrations of enzyme bound intermediates build up to their steady state levels. Once the intermediates reach their steady state concentrations (and this is generally achieved after milliseconds) the reaction rate changes only slowly with time. [Pg.157]

The results of Fink et al. (1983) demonstrate that in 70% methanol at sufficiently low temperatures, the binding of 2, 3 -CMP to RNase A still occurs relatively rapidly, but its turnover to product can be made negligible. This determination of the experimental conditions necessary to accumulate and stabilize this noncovalent enzyme-bound intermediate allows crystallographic studies of its structure to be carried out. These studies are described in Section V,C,2. [Pg.267]

Treatment of the reaction mixture with sodium borohydride-f followed by acidic hydrolysis led to major fractions of 6-deoxy-D-glucose-f and D-fucose-t, together with a minor fraction of D-galac-tose-4-f.422 This fact indicates that 109 is formed as an enzyme-bound intermediate, and allows the exclusion of a possible, alternative mechanism, shown in Fig. 3 as B, for the conversion of 107a into 108a through an intermediate glycos-5-ene derivative 111. [Pg.380]

This reaction occurs in two steps in the enzyme s active site. In step (Fig. 27-14) an enzyme-bound intermediate, aminoacyl adenylate (aminoacyl-AMP), forms when the carboxyl group of the amino acid reacts with the a-phosphoryl group of ATP to form an anhydride linkage, with displacement of pyrophosphate. In the sec-... [Pg.1051]

In one of the haloacid dehalogenases, a 232-residue protein for which the three-dimensional structure is known,8 9 Asp 10 is in a position to carry out the initial attack which would give an enzyme-bound intermediate with an ester linkage ... [Pg.590]

Identification of glycosyl-enzyme intermediates. Studies with pure enzymes often make it possible to confirm directly the existence of enzyme-bound intermediates. The intermediates detected are frequently glycosyl esters of glutamate or aspartate side chain... [Pg.595]

The result of equation 3.39 for nonproductive binding is quite general. It applies to cases in which intermediates occur on the reaction pathway as well as in the nonproductive modes. For example, in equation 3.19 for the action of chy-motrypsin on esters with accumulation of an acylenzyme, it is seen from the ratios of equations 3.21 and 3.22 that kQJKM = k2IKs. This relationship clearly breaks down for the Briggs-Haldane mechanism in which the enzyme-substrate complex is not in thermodynamic equilibrium with the free enzyme and substrates. It should be borne in mind that KM might be a complex function when there are several enzyme-bound intermediates in rapid equilibrium, as in equation 3.16. Here kcJKM is a function of all the bound species. [Pg.69]

Whether or not the physiologically relevant substrates involve the accumulation of an enzyme-bound intermediate at saturating substrate concentrations. It should be noted that if the physiological concentration of the substrate is below its KM value, an intermediate does not accumulate, even if it would at saturating concentrations. [Pg.198]

An even better way to determine absolute rate constants is to use pre - steady state kinetics to measure the rate constants for the formation or decay of enzyme-bound intermediates (Chapter 4). The rate constants for first-order exponential time courses are independent of enzyme concentration and so are unaffected by the presence of denatured enzyme. The impurity just lowers the amplitude of the trace. Pre-steady state kinetics are also less prone to artifacts, discussed next, that are caused by the presence of small amounts of contaminants that have a much higher activity than the mutant being analyzed. The steady state kinetics of a weakly active mutant could be dominated by a fraction of a percent of wild type. In pre-steady state kinetics, however, that contaminant would contribute only a fraction of a percent of the amplitude of the trace. This would be either lost in the noise or observed as a minor fast phase. [Pg.223]

Figure 15.3 Superposition of free energy profiles for wild-type (E) and mutant (E ) enzymes. The reaction involves the formation of an ES complex followed by a transition state for a chemical step, ES, and then the formation of an enzyme-bound intermediate El. Note that the free enzymes are arbitrarily assigned the same free energy. This is valid for comparing the changes in interaction energies at different stages of the reaction (see Chapter 3, section L3, and A. R. Fersht, A. Matouschek, and L.Serrano, J. Molec. Biol. 224,771 (1992)).

The calculation of rate constants from steady state kinetics and the determination of binding stoichiometries requires a knowledge of the concentration of active sites in the enzyme. It is not sufficient to calculate this specific concentration value from the relative molecular mass of the protein and its concentration, since isolated enzymes are not always 100% pure. This problem has been overcome by the introduction of the technique of active-site titration, a combination of steady state and pre-steady state kinetics whereby the concentration of active enzyme is related to an initial burst of product formation. This type of situation occurs when an enzyme-bound intermediate accumulates during the reaction. The first mole of substrate rapidly reacts with the enzyme to form stoichiometric amounts of the enzyme-bound intermediate and product, but then the subsequent reaction is slow since it depends on the slow breakdown of the intermediate to release free enzyme. [Pg.415]

There are circumstances in which the simple rules for the partition of intermediates break down. If the acceptor nucleophile reacts with the acylenzyme before the leaving group has diffused away from the enzyme-bound intermediate, the partition ratio could depend on the nature of the leaving group (e.g., due to Steric hindrance of attack, etc.). Also, the measurement of rate constants for the attack of the nucleophiles on the intermediate could be in slight error due to the nonspecific binding effects mentioned above. [Pg.453]

The prerequisite for protein engineering studies is that the enzyme has been cloned and expressed. Further, unless only relatively crude information is required, it is essential that the structure has been solved at high resolution. Accurate structure-activity studies require even more stringent criteria absolute values of rate constants. The two following procedures, which were discussed earlier (Chapter 4, section E), must be available. Both depend on the accumulation of an enzyme-bound intermediate or product on the reaction pathway. [Pg.549]

Assume that a mutant form of glyceraldehyde-3-phosphate dehydrogenase was found to hydrolyze the oxidized enzyme-bound intermediate with water rather than phosphate. [Pg.278]

The reaction catalyzed by ribulose bisphosphate carboxylase involves 2-carboxy-3-ketoarabinitol-1,5-bisphosphate as an enzyme-bound intermediate. The intermediate probably forms by the addition of C02 to the enolate of ribulose-1,5-bisphosphate. The substrate is known to be C02 rather than bicarbonate. [Pg.350]

Marquez LA, Dunford HB (1994) Chlorination of Taurine by Myeloperoxidase. Kinetic Evidence for an Enzyme-Bound Intermediate. J Biol Chem 269 7950... [Pg.490]

These are metabolites that bind primarily to the parent enzyme. This category includes substrates that form enzyme-bound intermediates that react with the active site of the enzyme. Such chemicals are known as suicide substrates. A number of compounds are known to react in this manner with CYP, and such compounds are often used experimentally as CYP inhibitors (see the discussion of piperonyl butoxide, Section 7.2.2). Other compounds, although not true suicide substrates, produce reactive metabolites that bind primarily to the activating enzyme or adjacent proteins altering the function of the protein. [Pg.151]

NOS catalyzes two sequential monooxygenase reactions. First, NOS hy-droxylates L-arginine (132) to generate an enzyme-bound intermediate, N-hydroxy-L-argininc (133). Then, 133 is further oxidized to generate nitric oxide ( NO) and citrulline (134) [128] (Scheme 29). [Pg.160]

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