Heterologous gene expression

The rapidly increasing number of novel metagenome-derived genes also led to the identiflcation of major bottlenecks. These are mainly linked to the eflhciency of heterologous gene expression and the amount of protein produced, in particular. [Pg.74]

Nijland, R., Veening, J.W. and Kuipers, O.P. (2007) A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression. Applied and Environmental Microbiology, 73 (7), 2390-2393. [Pg.54]

Here, we describe the various alternatives to the use of E. coli expression hosts for heterologous gene expression. Advantages and disadvantages for the different expression systems are discussed, and practical aspects of expression technologies are also described. The feasibility of isotope labeling of recombinantly expressed proteins and their potential use for NMR is also discussed, since the costs and quantities of recombinant proteins produced depend on the system being used. [Pg.21]

M. C. Heterologous gene expression in a membrane-protein-spedfic system. Protein Express. Lharif. 1999,... [Pg.281]

Overexpression of key genes of the synthetic pathways Heterologous gene expression/use of engineered enzymes Knockouts of genes involved in product degradation Precursor approach instead of de novo biosynthesis... [Pg.511]

Different AAV serotypes have shown remarkably different expression patterns because of differences in cell entry and intracellular activities (66, 67). Application of the dimerizer-inducible transcriptional regulatory system for AAV has allowed pharmacological regulation of heterologous gene expression in vivo (68). [Pg.342]

High levels of heterologous gene expression are often achieved by using the strong polyhedrin promoter of the Astographa californica nuclear polyhedrosis virus (AcNPV) ... [Pg.23]

Heterologous gene expression combined with targeted gene disruption (69,112) (Fig. lA, B). [Pg.214]

Plant virus-based vectors have a number of advantages as heterologous gene expression tools (for general reviews see Porta and Lomonossoff, 1996 Scholthof et al, 1996). These include the ability to direct rapid and... [Pg.14]

A heterologous gene expression system is chosen with regard to both its characteristics and the anticipated application of the expressed protein. Often, both economic and production issues play a major role in choosing a particular expression system. An ideal system provides both low cost and high production capacity. [Pg.16]

Glick, B.R. (1995) Metabolic load and heterologous gene expression. Biotechnol. Adv., 13, 247-261. [Pg.276]

The C-line Use of the Immunoglobulin Locus of a Human/Mouse Heterohybridoma for Heterologous Gene Expression... [Pg.769]

Swinkels B W, van Ooyen All, Bonekamp F L (1993). The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression. Antonie van Leeuwenhoek 64 187-201. [Pg.41]

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