With the advances in computer technology, graphical interfaces improve substantially software operability and readability. Good icon converts series of complex system commands into an intuitive symbol and enhances software usability by reducing users' cognitive load. In this paper, we study the semantics of universal symbols, the Time to Fixation Mean and Heat Map are carried out by the eye-tracking experiment, then the corresponding icons are designed according to the results of eye-tracking experiment, finally we analyze the efficiency (Reaction time and accuracy) of user's recognition in the e-prime experiment. This paper provides designer with a design method of icons after a quantitative verification.