The development of intelligent industrialization has gradually increased the demand for industrial robots, and it has also promoted the emergence and development of industrial robot protective clothing. At this stage, the design methods of sewing patterns are limited to planes and lack simulation stitching and try-on in three-dimensional space. In this study, the virtual try-on of protective clothing for industrial robots was realized in the CLO3D environment, and the fabric was simulated using tools in the software. First, import the digital model of the industrial robot into CLO3D, and copy the AUTO CAD template in CLO3D. Then, input the results of the fabric test to simulate the fabric. Use the software’s virtual stitching and virtual wearing to observe the wearing effect of protective clothing and the data-based fabric pressure, directly modify the model and observe the modification effect. After research, the three-dimensional simulated fitting can clearly show the characteristics of looseness or tightness and whether it is convenient to put on and take off. The model can be adjusted appropriately in time, which can reduce costs and improve production efficiency. It is an important exploration for the development of industrial robot protective clothing.