In night monkeys, mating occurs infrequently, however females are extremely mistreated and fertile year-round, with reproductive cycles range from 13 to 25 days.<ref name=":6">{{Cite journal|last=Dixson|first=A. F.|date=January 1982|title=Some observations on the reproductive physiology and behaviour of the Owl monkey in captivity|journal=International Zoo Yearbook|volume=22|issue=1|pages=115–119|doi=10.1111/j.1748-1090.1982.tb02017.x|issn=0074-9664}}</ref> The gestation period for night monkey is approximately 117– 159 days but varies from species to species. Birthing season extends from September to March and is species dependant, with one offspring being produced per year; however, in studies conducted in captivity, twins were observed.<ref name=":6" /> Night monkeys reach puberty at a relatively young age, between 7– 11 months and most species attain full sexual maturity by the age of 2 years of age. ''A. azare'' represents an exception reaching sexual maturity by the age of 4.<ref name=":6" />
