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{{short description|RNA molecule chain}}
'''Spiegelman Monster''' is the name given to an [[RNA]] chain of only 218 [[nucleotides]] that is able to be reproduced by an [[RNA replicase|RNA replication enzyme]]. It is named after its creator, [[Sol Spiegelman]], of the [[University of Illinois at Urbana-Champaign]].
'''Spiegelman's Monster''' is an [[RNA]] chain of only 218 [[nucleotides]] that is able to be reproduced by the RNA replication enzyme [[RNA-dependent RNA polymerase]], also called RNA replicase. It is named after its creator, [[Sol Spiegelman]], of the [[University of Illinois at Urbana-Champaign]] who first described it in 1965.


==Description==
==Description==
⚫
Spiegelman introduced RNA from a simple [[bacteriophage Qβ]] (Qβ) into a solution which contained Qβ's RNA replicase, some free nucleotides, and some salts. In this environment, the RNA started to be replicated.<ref>{{cite journal| author= Spiegelman, S., Haruna, I., Holland, I.B., Beaudreau, G. & Mills, D. |year=1965 |title=The Synthesis of a Self-propagating and Infectious Nucleic Acid with a Purified Enzyme | journal=Proc. Natl. Acad. Sci. USA |volume=54 |pages=919–927|pmc=219765 |pmid=5217468 |issue=3| doi= 10.1073/pnas.54.3.919|bibcode=1965PNAS...54..919S |doi-access=free }}</ref><ref>{{cite journal| doi=10.1073/pnas.58.1.217| title=An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule| journal=[[Proceedings of the National Academy of Sciences]]| author =Mills, D. R., R. L. Peterson, Sol Spiegelman| year=1967| volume=58| issue=1| pages=217–24| pmid=5231602| pmc=335620| bibcode=1967PNAS...58..217M| doi-access=free}}</ref> After a while, Spiegelman took some RNA and moved it to another tube with fresh solution. This process was repeated.<ref name="monster">{{cite journal| doi=10.1073/pnas.69.10.3038| title=A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication| journal=[[Proceedings of the National Academy of Sciences]]| author =Kacian,D.L., D.R. Mills, F.R. Kramer, S. Spiegelman| year=1972| pmid=4507621|volume=69| issue=10| pages=3038–3042| pmc=389702| bibcode=1972PNAS...69.3038K| doi-access=free}}</ref>
Spiegelman introduced RNA from a simple [[Bacteriophage Qβ]] (Qβ) into a solution which contained the RNA replication enzyme [[RNA replicase]] from the Qβ virus Q-Beta Replicase, some free nucleotides and some salts. In this environment, the RNA started to replicate.<ref>{{cite journal| author= Spiegelman, S., Haruna, I., Holland, I.B., Beaudreau, G.
⚫
& Mills, D. |year=1965 |title=The Synthesis of a Self-propagating and Infectious Nucleic Acid with a Purified Enzyme | journal=Proc. Nat. Acad. Sci. USA |volume=54 |pages=919–927|pmc=219765 |pmid=5217468 |issue=3}}</ref> After a while, Spiegelman took some RNA and moved it to another tube with fresh solution. This process was repeated.<ref name="monster">{{cite journal| doi=10.1073/pnas.69.10.3038| title=A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication| journal=[[Proceedings of the National Academy of Sciences]]| author =Kacian,D.L., D.R. Mills, F.R. Kramer, S. Spiegelman| year=1972| pmid=4507621| url=http://www.pnas.org/content/69/10/3038.abstract| volume=69| issue=10| pages=3038–3042| pmc=389702}}</ref>


Shorter RNA chains were able to replicate faster, so the RNA became shorter and shorter as selection favored speed. After 74 generations, the original strand with 4,500 nucleotide bases ended up as a dwarf genome with only 218 bases. Such a short RNA had been able to replicate very quickly in these unnatural circumstances.
Shorter RNA chains were able to be replicated faster, so the RNA became shorter and shorter as selection favored speed. After 74 generations, the original strand with 4,500 nucleotide bases ended up as a dwarf genome with only 218 bases. This short RNA sequence replicated very quickly in these unnatural circumstances.


== Further work ==
In 1997, [[Manfred Eigen|Eigen]] and Oehlenschlager showed that the Spiegelman monster eventually becomes even shorter, containing only 48 or 54 nucleotides, which are simply the binding sites for the reproducing enzyme [[RNA replicase]].<ref name="EIG">{{cite journal| title=30 Years Later – a New Approach to Sol Spiegelman's and [[Leslie Orgel]]'s in vitro EVOLUTIONARY STUDIES Dedicated to Leslie Orgel on the occasion of his 70th birthday| journal=Origins of Life and Evolution of Biospheres| last=Oehlenschläger| first=Frank| coauthors=Manfred Eigen| year=1997| doi=10.1023/A:1006501326129| volume=27| issue=5/6| pages=437}}</ref>
⚫
M. Sumper and R. Luce of [[Manfred Eigen]]'s laboratory replicated the experiment, except without adding RNA, only RNA [[Nucleobase|bases]] and Qβ replicase. They found that under the right conditions the Qβ replicase can spontaneously generate RNA which evolves into a form similar to Spiegelman's Monster.<ref>{{cite journal| doi=10.1073/pnas.72.1.162| title=Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase | journal=[[Proceedings of the National Academy of Sciences]] | last=Sumper | first=M |author2=R. Luce | year=1975| volume=72 | issue=1 | pages=162–166 | pmid=1054493| pmc=432262| bibcode=1975PNAS...72..162S | url=https://epub.uni-regensburg.de/22926/1/sumper45.pdf| doi-access=free }}</ref>


Eigen built on Spiegelman's work and produced a similar system further degraded to just 48 or 54 nucleotides—the minimum required for the binding of the replication enzyme, this time a combination of HIV-1 [[reverse transcriptase]] and [[T7 RNA polymerase]].<ref name="EIG">{{cite journal |last1= Oehlenschläger |first1= Frank |last2= Eigen |first2= Manfred |authorlink2= Manfred Eigen |date= December 1997 |title= 30 Years Later – a New Approach to Sol Spiegelman's and Leslie Orgel's in vitro Evolutionary Studies Dedicated to Leslie Orgel on the occasion of his 70th birthday |journal= [[Origins of Life and Evolution of Biospheres]] |volume= 27 |issue= 5–6 |pages= 437–457 |doi= 10.1023/A:1006501326129 |pmid= 9394469|bibcode= 1997OLEB...27..437O |s2cid= 26717033 }}</ref><ref name=“Ancestor”>{{cite book |title=The Ancestor's Tale |isbn=978-0544859937 |last1=Dawkins |first1=Richard |last2=Wong |first2=Yan |year=2016 |title-link=The Ancestor's Tale |publisher=Houghton Mifflin Harcourt |author1-link=Richard Dawkins }}</ref>
⚫
M. Sumper and R. Luce of Eigen's laboratory demonstrated that a mixture containing no RNA at all but only RNA [[Nucleobase|bases]] and Q-Beta Replicase can, under the right conditions, spontaneously generate self-replicating RNA which evolves into a form similar to Spiegelman's Monster.<ref>{{cite journal| doi=10.1073/pnas.72.1.162| title=Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase | journal=[[Proceedings of the National Academy of Sciences]] | last=Sumper | first=M | coauthors= R. Luce | year=1975| volume=72 | issue=1 | pages=162–166 | pmid=1054493| pmc=432262}}</ref>

⚫
== References ==
⚫
{{reflist}}


== See also ==
== See also ==
*[[Abiogenesis]]
*[[RNA world hypothesis]]
*[[RNA world hypothesis]]
*[[PAH world hypothesis]]
⚫
*[http://www.asa3.org/archive/asa/200001/0229.html ASA - January 2000: almost life]
*[[Viroid]]

⚫
== References ==
⚫
{{reflist|2}}

==External links==
⚫
*[http://www2.asa3.org/archive/asa/200001/0229.html ASA - January 2000: almost life]
*[http://www.numericana.com/answer/mystery.htm#life Not-so-Final Answers - The origin of life]
*[http://www.numericana.com/answer/mystery.htm#life Not-so-Final Answers - The origin of life]

*[http://math.ucr.edu/home/baez/subcellular.html Subcellular Life Forms]
{{Organisms et al.}}


[[Category:Origin of life]]
[[Category:Origin of life]]
[[Category:RNA]]
[[Category:RNA]]
[[Category:Molecular evolution]]

[[ca:Monstre de Spiegelman]]
[[ru:Монстр Шпигельмана]]
[[fi:Spiegelmanin hirviö]]

Revision as of 19:59, 22 September 2024

Spiegelman's Monster is an RNA chain of only 218 nucleotides that is able to be reproduced by the RNA replication enzyme RNA-dependent RNA polymerase, also called RNA replicase. It is named after its creator, Sol Spiegelman, of the University of Illinois at Urbana-Champaign who first described it in 1965.

Description

Spiegelman introduced RNA from a simple bacteriophage Qβ (Qβ) into a solution which contained Qβ's RNA replicase, some free nucleotides, and some salts. In this environment, the RNA started to be replicated.[1][2] After a while, Spiegelman took some RNA and moved it to another tube with fresh solution. This process was repeated.[3]

Shorter RNA chains were able to be replicated faster, so the RNA became shorter and shorter as selection favored speed. After 74 generations, the original strand with 4,500 nucleotide bases ended up as a dwarf genome with only 218 bases. This short RNA sequence replicated very quickly in these unnatural circumstances.

Further work

M. Sumper and R. Luce of Manfred Eigen's laboratory replicated the experiment, except without adding RNA, only RNA bases and Qβ replicase. They found that under the right conditions the Qβ replicase can spontaneously generate RNA which evolves into a form similar to Spiegelman's Monster.[4]

Eigen built on Spiegelman's work and produced a similar system further degraded to just 48 or 54 nucleotides—the minimum required for the binding of the replication enzyme, this time a combination of HIV-1 reverse transcriptase and T7 RNA polymerase.[5][6]

See also

References

  1. ^ Spiegelman, S., Haruna, I., Holland, I.B., Beaudreau, G. & Mills, D. (1965). "The Synthesis of a Self-propagating and Infectious Nucleic Acid with a Purified Enzyme". Proc. Natl. Acad. Sci. USA. 54 (3): 919–927. Bibcode:1965PNAS...54..919S. doi:10.1073/pnas.54.3.919. PMC 219765. PMID 5217468.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. ^ Mills, D. R., R. L. Peterson, Sol Spiegelman (1967). "An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule". Proceedings of the National Academy of Sciences. 58 (1): 217–24. Bibcode:1967PNAS...58..217M. doi:10.1073/pnas.58.1.217. PMC 335620. PMID 5231602.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  3. ^ Kacian,D.L., D.R. Mills, F.R. Kramer, S. Spiegelman (1972). "A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication". Proceedings of the National Academy of Sciences. 69 (10): 3038–3042. Bibcode:1972PNAS...69.3038K. doi:10.1073/pnas.69.10.3038. PMC 389702. PMID 4507621.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. ^ Sumper, M; R. Luce (1975). "Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase" (PDF). Proceedings of the National Academy of Sciences. 72 (1): 162–166. Bibcode:1975PNAS...72..162S. doi:10.1073/pnas.72.1.162. PMC 432262. PMID 1054493.
  5. ^ Oehlenschläger, Frank; Eigen, Manfred (December 1997). "30 Years Later – a New Approach to Sol Spiegelman's and Leslie Orgel's in vitro Evolutionary Studies Dedicated to Leslie Orgel on the occasion of his 70th birthday". Origins of Life and Evolution of Biospheres. 27 (5–6): 437–457. Bibcode:1997OLEB...27..437O. doi:10.1023/A:1006501326129. PMID 9394469. S2CID 26717033.
  6. ^ Dawkins, Richard; Wong, Yan (2016). The Ancestor's Tale. Houghton Mifflin Harcourt. ISBN 978-0544859937.
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