ViroPure RNA Isolation Kit

Catalogue No : VP-1001

Related applications : Viral RNA Isolation

Catalog NoUnit SizePrice (INR)
*VP-1001-5050Contact us
*VP-1001-100100Contact us
*VP-1001-200200Contact us
*VP-1001-500500Contact us
Product Overview     Documents     

Description

ViroPure RNA isolation kit is designed for purification of high quality viral RNA from serum, plasma (treated with non-heparin anticoagulants), cell culture supernatant, human and animal derived liquid samples such as nasal swab, throat swab and urogenital swab. 

The kit works on silica membrane technology that takes into account the nucleic acid binding property of silica under highly denaturing conditions provided by chaotrophic salts. The presence of strong denaturants also ensure the inactivation of RNAse present in the sample, which facilitates the recovery of intact RNA. The kit is designed in such a way to purify nucleic acid larger than 200 nucleotide, therefore the nucleic acid strand smaller than this size will be excluded. The kit is not designed to exclude DNA from isolated RNA, which generally does not interferes in downstream applications. If required to remove DNA from RNA, then users are advised to follow DNAse based procedure from respective supplier.

 

 Specifications

Sample InputSerum, Plasma (treated with non-heparin anticoagulants), cell culture supernatant, human and animal derived liquid samples such as nasal swab, throat swab and urogenital swab
SensitivityLower limit: 1-100 copies
Compatibility downstreamRT-qPCR, TaMan Based RT-qPCR Assays
Yield1-2 ug depending on Virus titer
Highlights
  • Technically approved by Indian council of Medical Research
  • Tested on nasal and throat swab from COVID-19 positive sample by ICMR
  • RNA quality and purity are equivalent or better than already available viral RNA isolation kit from reputed supplier
  • No interference in downstream RT-PCR based diagnostic assays detected

 

 
 
Content and Storage

* Spin columns, Collection Tubes, Buffer VL, Buffer W1(concentrated), Buffer W2(concentrated), Elution buffer, Carrier RNA

Storage:  Room temperature

 

Related Product

*  Cubexa Gel extraction Kit

*  Cubexa Plasmid purification Kit

*  Cubexa PCR clean up kit

 

Figures

Documents

 
Manuals and Protocols

User guide : ViroPure RNA Isolation manual

Material Safety Datasheets (MSDS)

#VRIM-101