Figure 1.
Expression of conserved tomato miRNAs. Total RNA from different tissues was extracted, separated, and transferred to membranes. The membranes were hybridized to miRNA-specific probes or a U6-specific probe (shown on the right) to demonstrate equal loading. Membranes were stripped and reprobed; equal loading is shown once for each membrane. Numbers between brackets indicate the number of sequences found in the fruit (left) and leaf (right) libraries for each miRNA. Different size fruits were used for RNA extraction: F1, 1–3 mm; F2, 5–7 mm; F3, 7–11 mm; F4, 11–14 mm. Size markers on the left are 24- and 19-nt RNA oligonucleotides.
