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The experimental results demonstrated that this robotic enucleation method is capable of enucleation with less time-cost, higher enucleation accuracy, higher enucleation success rate, and higher cleavage rate compared with the manual method.
Conclusions: The results prove that our method significantly improves the developmental competence of cloned embryos in comparison to manual enucleation method.
Oocyte enucleation (OE) is a requisite step in animal clone. The traditional OE manipulation requires high professional skills and involves contaminations ...
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What is oocyte enucleation?
Are oocytes and ovums the same?
During oocyte enucleation, the external force is exerted on the oocyte by the injection micropipette and injector, in which huge deformations of the oocyte are ...
A single oocyte enucleation can be completed in less than 13 seconds. This robotic enucleation method provides a solution for enhancing the operational ...
Feb 19, 2021 · In this paper, we developed a robotic precise oocyte blind enucleation method to precisely control the removed cytoplasm during enucleation and ...
Enucleation is a crucial step in cloning. In order to achieve automatic blind enucleation, we should detect the polar body of the oocyte automatically.
We propose a novel on-chip enucleation of an oocyte with zona pellucida by using a combination of untethered microrobots.
We developed a highly functional untethered micro-robot that can manipulate cells with high gripping force in a micro-fluidic chip. The robot has gripping ...
This paper presents on-chip enucleation of oocyte by the robot integrated microfluidic chip (Robochip). Enucleation process is mainly divided in three ...