Papers by Rasheena Edmondson
MAPK inhibition only slightly decreases the migratory capacity of GBM cells. In conclusion, the r... more MAPK inhibition only slightly decreases the migratory capacity of GBM cells. In conclusion, the results of this study collectively suggest that: (1) Fra-1 and CD44 expression are regulated by the MAPK and/or AKT/mTOR signal transduction pathways in GBM; (2) Fra-1 regulates CD44 expression in human GBM cells, which in turn affects glioma cell migration.
PLOS ONE, Jun 28, 2016
This study investigated the effects of matrix on the behaviors of 3D-cultured cells of two prosta... more This study investigated the effects of matrix on the behaviors of 3D-cultured cells of two prostate cancer cell lines, LNCaP and DU145. Two biologically-derived matrices, Matrigel and Cultrex BME, and one synthetic matrix, the Alvetex scaffold, were used to culture the cells. The cell proliferation rate, cellular response to anti-cancer drugs, and expression levels of proteins associated with drug sensitivity/resistance were examined and compared amongst the 3D-cultured cells on the three matrices and 2D-cultured cells. The cellular responses upon treatment with two common anti-cancer drugs, Docetaxel and Rapamycin, were examined. The expressions of epidermal growth factor receptor (EGFR) and β-III tubulin in DU145 cells and p53 in LNCaP cells were examined. The results showed that the proliferation rates of cells cultured on the three matrices varied, especially between the synthetic matrix and the biologically-derived matrices. The drug responses and the expressions of drug sensitivity-associated proteins differed between cells on various matrices as well. Among the 3D cultures on the three matrices, increased expression of β-III tubulin in DU145 cells was correlated with increased resistance to Docetaxel, and decreased expression of EGFR in DU145 cells was correlated with increased sensitivity to Rapamycin. Increased expression of a p53 dimer in 3D-cultured LNCaP cells was correlated with increased resistance to Docetaxel. Collectively, the results showed that the matrix of 3D cell culture models strongly influences cellular behaviors, which highlights the imperative need to achieve standardization of 3D cell culture technology in order to be used in drug screening and cell biology studies.
RSC Advances, 2020
The antiviral function of carbon dots (CDots) with visible light exposure was evaluated, for whic... more The antiviral function of carbon dots (CDots) with visible light exposure was evaluated, for which the model bacteriophages MS2 as a surrogate of small RNA viruses were used. The results show clearly that the visible light-activated CDots are highly effective in diminishing the infectivity of MS2 in both low and high titer samples to the host E. coli cells, and the antiviral effects are dot concentration-and treatment timedependent. The action of CDots apparently causes no significant damage to the structural integrity and morphology of the MS2 phage or the breakdown of the capsid proteins, but does result in the protein carbonylation (a commonly used indicator for protein oxidation) and the degradation of viral genomic RNA. Mechanistically the results may be understood in the framework of photodynamic effects that are associated with the unique excited state properties and processes of CDots. Opportunities for potentially broad applications of CDots coupled with visible/natural light in the prevention and control of viral transmission and spread are highlighted and discussed.
Survival of 3 Day and 5 Day spheroids after Docetaxel treatment
<p>(A) DU145 and (B) LNCaP cells were grown for 3 or 5 days, followed by Docetaxel treatmen... more <p>(A) DU145 and (B) LNCaP cells were grown for 3 or 5 days, followed by Docetaxel treatment for 48h at a concentration of 5 μM and 0.07 μM, respectively. Cell survival was calculated as a percentage of the ratio between treated and untreated cells (Cell Survival %). Data is expressed as mean ± SEM based on at least three independent experiments with triplicate wells for each drug concentration.</p
Fluorescent microscopic images of DU145 and LNCaP cells cultured in the 3D matrices: Matrigel, BME, and Alvetex
<p>Cells were seeded onto the various matrices and cultured for four days. Hoechst 33342 (b... more <p>Cells were seeded onto the various matrices and cultured for four days. Hoechst 33342 (blue) and CellTracker Red CMPTX dye (red) was used to stain nuclei and cytoplasm, respectively. Images obtained using BD Pathway Bioimager.</p
Prostate cancer proliferation of 3D- and 2D-cultured cells
<p>(A) DU145 and (B) LNCaP cells were grown in 3D matrices (Alvetex, Matrigel, and BME) and... more <p>(A) DU145 and (B) LNCaP cells were grown in 3D matrices (Alvetex, Matrigel, and BME) and 2D culture. Cell viability measured at 72 and 120 h of growth. Initial cell density was 5,000 cells per well. Results obtained using CellTiter-Glo 3D cell viability assay. Asterisks represent a significant difference between groups. *p≤0.05 using one-way ANOVA and unpaired t-tests.</p
Total EGFR and β-III tubulin protein expression in DU145 cells
<p>A representative image of total p53 protein expression in 2D- and 3D-cultured LNCaP cell... more <p>A representative image of total p53 protein expression in 2D- and 3D-cultured LNCaP cells. Bars represent the relative protein quantification of p53 isoforms at ~144, ~100, ~58, and ~44 kda on the basis of β-actin, and indicate the mean ± SEM (n = 3 for each group). *p≤0.05 using one-way ANOVA and unpaired t-tests.</p
Optical microscopic images of DU145 and LNCaP spheroids in Matrigel and BME after 3 day and 5 day growth period
<p>(A) DU145 and (B) LNCaP cells were seeded onto Matrigel- and BME- coated wells and grown... more <p>(A) DU145 and (B) LNCaP cells were seeded onto Matrigel- and BME- coated wells and grown for 3 or 5 days. Scale bars indicate the width of spheroid, and are shown at 25 and 50 μm.</p
MAPK inhibition only slightly decreases the migratory capacity of GBM cells. In conclusion, the r... more MAPK inhibition only slightly decreases the migratory capacity of GBM cells. In conclusion, the results of this study collectively suggest that: (1) Fra-1 and CD44 expression are regulated by the MAPK and/or AKT/mTOR signal transduction pathways in GBM; (2) Fra-1 regulates CD44 expression in human GBM cells, which in turn affects glioma cell migration.
PLOS ONE, 2016
This study investigated the effects of matrix on the behaviors of 3D-cultured cells of two prosta... more This study investigated the effects of matrix on the behaviors of 3D-cultured cells of two prostate cancer cell lines, LNCaP and DU145. Two biologically-derived matrices, Matrigel and Cultrex BME, and one synthetic matrix, the Alvetex scaffold, were used to culture the cells. The cell proliferation rate, cellular response to anti-cancer drugs, and expression levels of proteins associated with drug sensitivity/resistance were examined and compared amongst the 3D-cultured cells on the three matrices and 2D-cultured cells. The cellular responses upon treatment with two common anti-cancer drugs, Docetaxel and Rapamycin, were examined. The expressions of epidermal growth factor receptor (EGFR) and β-III tubulin in DU145 cells and p53 in LNCaP cells were examined. The results showed that the proliferation rates of cells cultured on the three matrices varied, especially between the synthetic matrix and the biologically-derived matrices. The drug responses and the expressions of drug sensitivity-associated proteins differed between cells on various matrices as well. Among the 3D cultures on the three matrices, increased expression of β-III tubulin in DU145 cells was correlated with increased resistance to Docetaxel, and decreased expression of EGFR in DU145 cells was correlated with increased sensitivity to Rapamycin. Increased expression of a p53 dimer in 3D-cultured LNCaP cells was correlated with increased resistance to Docetaxel. Collectively, the results showed that the matrix of 3D cell culture models strongly influences cellular behaviors, which highlights the imperative need to achieve standardization of 3D cell culture technology in order to be used in drug screening and cell biology studies.
Journal of Analytical & Bioanalytical Techniques, 2015
This study systematically investigated the cell proliferation rates, spheroid structures, cellula... more This study systematically investigated the cell proliferation rates, spheroid structures, cellular responses to different anti-cancer drugs, the expression of drug action-related proteins, and the possible correlations among these properties of 3D spheroids on Matrigel in comparison to 2D monolayer cells, using two cancer cell lines-the prostate cancer cell line, DU145, and the oral cancer cell line, CAL27. Compared to the traditional 2D-cultured cells, 3D-cultured CAL27 cells had enhanced proliferation by approximately 50-70% at various seeding cell densities, whereas 3D-cultured DU145 cells showed reduced proliferation at all tested seeding cell densities by 20-40%. In drug tests, the sensitivity of 3D-cultured DU145 cells relative to 2D-cultured cells showed an obvious drug action mechanism dependency in response to three anticancer drugs, Rapamycin, Docetaxel, and Camptothecin, whereas 3D-cultured CAL27 cells responded more sensitively than 2D-cultured cells to all three tested drugs, Docetaxel, Bleomycin, and Erlotinib, indicating the relative proliferation rate between 3D and 2D cultured cells may be a dominating factor in this case and mitigated the factor of drug action mechanism. The elevated expression of EGFR in 3D-cultured CAL27 was correlated with its more sensitive response to Erlotinib (acting through binding to EGRF) compared to 2D-cultured cells; Similarly, the expression of βIII tubulin in 3D-cultured DU145 cells was found to be increased and correlated with their higher resistance to Doxetaxel compared to 2D-cultured cells.
ASSAY and Drug Development Technologies, 2014
Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and... more Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests. In this review, we discuss the characteristics of 3D cell culture systems in comparison to the two-dimensional (2D) monolayer culture, focusing on cell growth conditions, cell proliferation, population, and gene and protein expression profiles. The innovations and development in 3D culture systems for drug discovery over the past 5 years are also reviewed in the article, emphasizing the cellular response to different classes of anticancer drugs, focusing particularly on similarities and differences between 3D and 2D models across the field. The progression and advancement in the application of 3D cell cultures in cell-based biosensors is another focal point of this review.
RSC Advances, 2020
The antiviral function of carbon dots (CDots) with visible light exposure was evaluated, for whic... more The antiviral function of carbon dots (CDots) with visible light exposure was evaluated, for which the model bacteriophages MS2 as a surrogate of small RNA viruses were used.
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Papers by Rasheena Edmondson