Squalene sintasi
enzima
Squalene sintasi è un enzima microsomiale (numero EC 2.5.1.21[1]) appartenente alla classe delle transferasi, che catalizza la trasformazione di due molecole di farnesil pirofosfato in squalene
| Squalene sintasi | |
|---|---|
 Squalene sintasi umana legata a un inibitore | |
| Numero EC | 2.5.1.21 |
| Classe | Transferasi |
| Altri nomi | |
| Farnesil-difosfato farnesiltransferasi | |
| Banche dati | BRENDA, EXPASY, GTD, PDB (RCSB PDB PDBe PDBj PDBsum) |
| Fonte: IUBMB | |
- presqualene pirofosfato + NAD(P)H + H+ ⇄ squalene + pirofosfato + NAD(P)+
Note
modificaBibliografia
modifica- (EN) Kuswick-Rabiega, G. e Rilling, H.C. Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities. J. Biol. Chem. 262 (1987) 1505-1509. [PMID 3805037]
- (EN) Ericsson, J., Appelkvist, E.L., Thelin, A., Chojnacki, T. e Dallner, G. Isoprenoid biosynthesis in rat liver peroxisomes. Characterization of cis-prenyltransferase and squalene synthetase. J. Biol. Chem. 267 (1992) 18708-18714. [PMID 1527001]
- (EN) Tansey, T.R. e Shechter, I. Structure and regulation of mammalian squalene synthase. Biochim. Biophys. Acta 1529 (2000) 49-62. [PMID 11111077]
- (EN) LoGrasso, P.V., Soltis, D.A. e Boettcher, B.R. Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase. Arch. Biochem. Biophys. 307 (1993) 193-199. [PMID 8239656]
- (EN) Shechter, I., Klinger, E., Rucker, M.L., Engstrom, R.G., Spirito, J.A., Islam, M.A., Boettcher, B.R. e Weinstein, D.B. Solubilization, purification, and characterization of a truncated form of rat hepatic squalene synthetase. J. Biol. Chem. 267 (1992) 8628-8635. [PMID 1569107]
- (EN) Agnew, W.S. e Popják, G. Squalene synthetase. Stoichiometry and kinetics of presqualene pyrophosphate and squalene synthesis by yeast microsomes. J. Biol. Chem. 253 (1978) 4566-4573. [PMID 26684]
- (EN) Pandit, J., Danley, D.E., Schulte, G.K., Mazzalupo, S., Pauly, T.A., Hayward, C.M., Hamanaka, E.S., Thompson, J.F. e Harwood, H.J., Jr. Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis. J. Biol. Chem. 275 (2000) 30610-30617. [PMID 10896663]
- (EN) Radisky, E.S. e Poulter, C.D. Squalene synthase: steady-state, pre-steady-state, and isotope-trapping studies. Biochemistry 39 (2000) 1748-1760. [PMID 10677224]
