Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1989 Oct;33(10):1824–1826. doi: 10.1128/aac.33.10.1824

Interaction of biofilm bacteria with antibiotics in a novel in vitro chemostat system.

H Anwar 1, T van Biesen 1, M Dasgupta 1, K Lam 1, J W Costerton 1
PMCID: PMC172765  PMID: 2511804

Abstract

Pseudomonas aeruginosa was cultivated at low growth rates under iron-limiting conditions on acrylic tiles. Biofilm cells exhibited increased tobramycin resistance compared with that of planktonic cells, and in old biofilms were more resistant than were cells in young biofilms. However, on suspension of the biofilm bacteria, glycocalyx-mediated resistance was lost.

Full text

PDF
1824

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown M. R., Williams P. The influence of environment on envelope properties affecting survival of bacteria in infections. Annu Rev Microbiol. 1985;39:527–556. doi: 10.1146/annurev.mi.39.100185.002523. [DOI] [PubMed] [Google Scholar]
  2. Costerton J. W., Cheng K. J., Geesey G. G., Ladd T. I., Nickel J. C., Dasgupta M., Marrie T. J. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 1987;41:435–464. doi: 10.1146/annurev.mi.41.100187.002251. [DOI] [PubMed] [Google Scholar]
  3. Costerton J. W., Lam J., Lam K., Chan R. The role of the microcolony mode of growth in the pathogenesis of Pseudomonas aeruginosa infections. Rev Infect Dis. 1983 Nov-Dec;5 (Suppl 5):S867–S873. doi: 10.1093/clinids/5.supplement_5.s867. [DOI] [PubMed] [Google Scholar]
  4. Costerton J. W. Structure and plasticity at various organization levels in the bacterial cell. Can J Microbiol. 1988 Apr;34(4):513–521. doi: 10.1139/m88-088. [DOI] [PubMed] [Google Scholar]
  5. Kadurugamuwa J. L., Anwar H., Brown M. R., Hengstler B., Kunz S., Zak O. Influence of cephalosporins and iron on surface protein antigens of Klebsiella pneumoniae in vivo. Antimicrob Agents Chemother. 1988 Mar;32(3):364–368. doi: 10.1128/aac.32.3.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kadurugamuwa J. L., Anwar H., Brown M. R., Shand G. H., Ward K. H. Media for study of growth kinetics and envelope properties of iron-deprived bacteria. J Clin Microbiol. 1987 May;25(5):849–855. doi: 10.1128/jcm.25.5.849-855.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kadurugamuwa J. L., Anwar H., Brown M. R., Zak O. Effect of subinhibitory concentrations of cephalosporins on surface properties and siderophore production in iron-depleted Klebsiella pneumoniae. Antimicrob Agents Chemother. 1985 Feb;27(2):220–223. doi: 10.1128/aac.27.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keevil C. W., Bradshaw D. J., Dowsett A. B., Feary T. W. Microbial film formation: dental plaque deposition on acrylic tiles using continuous culture techniques. J Appl Bacteriol. 1987 Feb;62(2):129–138. doi: 10.1111/j.1365-2672.1987.tb02390.x. [DOI] [PubMed] [Google Scholar]
  9. Kunin C. M., Steele C. Culture of the surfaces of urinary catheters to sample urethral flora and study the effect of antimicrobial therapy. J Clin Microbiol. 1985 Jun;21(6):902–908. doi: 10.1128/jcm.21.6.902-908.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nickel J. C., Gristina A. G., Costerton J. W. Electron microscopic study of an infected Foley catheter. Can J Surg. 1985 Jan;28(1):50-1, 54. [PubMed] [Google Scholar]
  11. Nickel J. C., Ruseska I., Wright J. B., Costerton J. W. Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob Agents Chemother. 1985 Apr;27(4):619–624. doi: 10.1128/aac.27.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ombaka E. A., Cozens R. M., Brown M. R. Influence of nutrient limitation of growth on stability and production of virulence factors of mucoid and nonmucoid strains of Pseudomonas aeruginosa. Rev Infect Dis. 1983 Nov-Dec;5 (Suppl 5):S880–S888. doi: 10.1093/clinids/5.supplement_5.s880. [DOI] [PubMed] [Google Scholar]
  13. Prosser B. L., Taylor D., Dix B. A., Cleeland R. Method of evaluating effects of antibiotics on bacterial biofilm. Antimicrob Agents Chemother. 1987 Oct;31(10):1502–1506. doi: 10.1128/aac.31.10.1502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shand G. H., Anwar H., Kadurugamuwa J., Brown M. R., Silverman S. H., Melling J. In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun. 1985 Apr;48(1):35–39. doi: 10.1128/iai.48.1.35-39.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith H. Microbial surfaces in relation to pathogenicity. Bacteriol Rev. 1977 Jun;41(2):475–500. doi: 10.1128/br.41.2.475-500.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ward K. H., Anwar H., Brown R. W., Wale J., Gowar J. Antibody response to outer-membrane antigens of Pseudomonas aeruginosa in human burn wound infection. J Med Microbiol. 1988 Nov;27(3):179–190. doi: 10.1099/00222615-27-3-179. [DOI] [PubMed] [Google Scholar]
  17. Warren J. W., Muncie H. L., Jr, Bergquist E. J., Hoopes J. M. Sequelae and management of urinary infection in the patient requiring chronic catheterization. J Urol. 1981 Jan;125(1):1–8. doi: 10.1016/s0022-5347(17)54874-0. [DOI] [PubMed] [Google Scholar]
  18. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weinberg E. D. Iron withholding: a defense against infection and neoplasia. Physiol Rev. 1984 Jan;64(1):65–102. doi: 10.1152/physrev.1984.64.1.65. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES