Laser tweezers is suitable for manipulation of a single microscopic biological object. It can manipulate micro bioobject without contact in the closed space. Single cell manipulation is important for biological research, and 3D 6DOF manipulation (position control and orientation control) is useful technique in many biological experiments. In this paper we propose 3D synchronized laser manipulation system that can manipulate multiple micro-objects along separate trajectories in 3D space. The position as well as the orientation of microbeads can be controlled using this system. We succeeded in achieving orientation control of the microbead by using the laser trapped microtools. We demonstrate experimentally 3D 6DOF manipulation of microbeads