Copper is essential for life, but is toxic in excess. Copper homeostasis is achieved in the
cytoplasm and the periplasm as a unique feature of Gram-negative bacteria. Especially, it
has become clear the role of the periplasm and periplasmic proteins regarding whole-cell
copper homeostasis. Here, we addressed the role of the periplasm and periplasmic proteins
in copper homeostasis using a Systems Biology approach integrating experiments with
models. Our analysis shows that most of the copper-bound molecules localize in the …