Frequently we produce mispronunciation and hesitation in phonation due to the difficulty in pronunciation. Characteristics of such troubles in phonation, however, have not been clearly understood. This paper analyzes characteristics in Japanese tongue twister statistically and the characteristics of mispronunciation and hesitation in phonation by employing questionnaire. Employed speech samples are Japanese tongue twister, because we consider that tongue twister has many factors of mispronunciation and hesitation. The results of questionnaire show that hesitation frequently occurs at vowels, particularly at vowel/a/. Palatalized sounds are found many times in the part of mispronunciation and hesitation. These results will contribute to improve the quality of speech synthesized by rule.