This study aims to grasp the differences in user characteristics related to user interface (UI) design from the perspective of cultural differences and examine how to approach UI design. This study mainly focused on the cultural differences between the United States and Japan to examine UI design that takes cultural differences into account. Based on the following six cultural differences between the United States and Japan, differences in user characteristics related to UI design were examined, and how to approach UI design considering cultural differences was examined: (1) short-term or long-term orientation, (2) individualism or totalism, (3) having control of the environment or acceptance of a given environment, (4) preferring change or being risk-averse and seeking for stability, (5) emphasizing the value of time or the value of human relation, and (6) low- or high-context culture.