Assessing vertebrate biodiversity in a kelp forest ecosystem using environmental DNA
JA Port, JL O'Donnell, OC Romero‐Maraccini… - Molecular …, 2016 - Wiley Online Library
Preserving biodiversity is a global challenge requiring data on species’ distribution and
abundance over large geographic and temporal scales. However, traditional methods to survey …
abundance over large geographic and temporal scales. However, traditional methods to survey …
Using environmental DNA to census marine fishes in a large mesocosm
The ocean is a soup of its resident species' genetic material, cast off in the forms of metabolic
waste, shed skin cells, or damaged tissue. Sampling this environmental DNA (eDNA) is a …
waste, shed skin cells, or damaged tissue. Sampling this environmental DNA (eDNA) is a …
Harnessing DNA to improve environmental management
Responsive environmental policy demands a constant stream of information about the living
world, but biological monitoring is difficult and expensive. For many species and ecosystems…
world, but biological monitoring is difficult and expensive. For many species and ecosystems…
Indexed PCR primers induce template-specific bias in large-scale DNA sequencing studies
JL O'Donnell, RP Kelly, NC Lowell, JA Port - PloS one, 2016 - journals.plos.org
Massively parallel sequencing is rapidly emerging as an efficient way to quantify biodiversity
at all levels, from genetic variation and expression to ecological community assemblage. …
at all levels, from genetic variation and expression to ecological community assemblage. …
Metagenomic frameworks for monitoring antibiotic resistance in aquatic environments
JA Port, AC Cullen, JC Wallace, MN Smith… - Environmental …, 2014 - ehp.niehs.nih.gov
Background: High-throughput genomic technologies offer new approaches for
environmental health monitoring, including metagenomic surveillance of antibiotic resistance …
environmental health monitoring, including metagenomic surveillance of antibiotic resistance …
Metagenomic profiling of microbial composition and antibiotic resistance determinants in Puget Sound
JA Port, JC Wallace, WC Griffith, EM Faustman - PloS one, 2012 - journals.plos.org
Human-health relevant impacts on marine ecosystems are increasing on both spatial and
temporal scales. Traditional indicators for environmental health monitoring and microbial risk …
temporal scales. Traditional indicators for environmental health monitoring and microbial risk …
FARME DB: a functional antibiotic resistance element database
JC Wallace, JA Port, MN Smith, EM Faustman - Database, 2017 - academic.oup.com
Antibiotic resistance (AR) is a major global public health threat but few resources exist that
catalog AR genes outside of a clinical context. Current AR sequence databases are …
catalog AR genes outside of a clinical context. Current AR sequence databases are …
Identification of G protein-coupled receptor signaling pathway proteins in marine diatoms using comparative genomics
JA Port, MS Parker, RB Kodner, JC Wallace… - BMC genomics, 2013 - Springer
Background The G protein-coupled receptor (GPCR) signaling pathway plays an essential
role in signal transmission and response to external stimuli in mammalian cells. Protein …
role in signal transmission and response to external stimuli in mammalian cells. Protein …
Variability in metagenomic samples from the Puget Sound: Relationship to temporal and anthropogenic impacts
JC Wallace, JE Youngblood, JA Port, AC Cullen… - PLoS …, 2018 - journals.plos.org
Whole-metagenome sequencing (WMS) has emerged as a powerful tool to assess potential
public health risks in marine environments by measuring changes in microbial community …
public health risks in marine environments by measuring changes in microbial community …
Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels
Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or
extracellular DNA, offers the opportunity to survey the biological composition of communities …
extracellular DNA, offers the opportunity to survey the biological composition of communities …