Urban areas can be considered the ground for the challenges related to the UN’s Sustainable Development Goals (SDGs). The objective of shaping cities as human settlement that will see a more inclusive, safe, resilient, and sustainable future is often argued in literature as an issue dependent on behavioral change of inhabitants in urban areas. In this paper, the authors question if experimental applications based on gamification can co-produce more sustainable neighborhoods through an impact evaluation method that departs from individual choices within the complex of urban mobility. This investigation is carried out within MUV (Mobility Urban Values), an EU research and innovation project, which aims to trigger more sustainable urban mobility in six pilot cities. This article describes the critical method of validation, an impact assessment of the MUV experimental gamification in the pilot cities, in order to represent a proof for future urban strategies. This methodological approach is based on an evaluation structured on indicators of both impact and process suitable for urban contexts. As based on six pilot cities, with possibilities for transferability to other contexts and scalability to other cities, the method represents a reference work for the evaluation of similar experimental applications.