This paper proposes a service differentiation architecture using virtualization of mobile femtocell. Network-prefix division multiple access (NDMA) is proposed to enable mobile devices inside a moving space to access the Internet with different service demands. With the NDMA scheme, our architecture provides multiple virtual mobile networks for diverse services which are optimized under different network conditions. We experimented on the operation of the proposed architecture with testbed implementation. The result shows the ability of multiple degrees of QoS over each virtual mobile network with additional benefits of personalization, mobility management aggregation, and so on.