The promoter hypermethylation of DAPK gene and p16 gene in non-small cell lung cancer patients
S Zheng, D Jiang, J Ge - 2009 2nd International Conference on …, 2009 - ieeexplore.ieee.org
S Zheng, D Jiang, J Ge
2009 2nd International Conference on Biomedical Engineering and …, 2009•ieeexplore.ieee.orgObjective: To evaluate the clinical significance of the aberrant methylation of DAPK gene
and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou
university. Methods: A methylation-specific PCR (MSP) was performed for the detection of
promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of
NSCLC. and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene
and the clinicopathological data. Results: 30.8%(20/65) of the sera from 65 cases of NSCLC …
and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou
university. Methods: A methylation-specific PCR (MSP) was performed for the detection of
promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of
NSCLC. and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene
and the clinicopathological data. Results: 30.8%(20/65) of the sera from 65 cases of NSCLC …
Objective
To evaluate the clinical significance of the aberrant methylation of DAPK gene and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou university.
Methods
A methylation-specific PCR (MSP)was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC. and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene and the clinicopathological data.
Results
30.8% (20 / 65)of the sera from 65 cases of NSCLC showed hypermethylation for DAPK promoter and 43.1 % (28 / 65)the same for p16 promoter, whereas no methylated DAPK gene promoter and p16 gene promoter were found in sera from the patients with lung benign diseases and normal controls. Methylated DAPK gene promoter and p16 gene promoter in sera were not closely correlated with the pathological classification , stage , metastas is and differentiation in NSCLC .
Conclusion
Detection of the aberrant methylation of DAPK gene and p16 gene in blood DNA from NSCLC patients might offer an effectivemeaa-lS for the earlier auxiliary diagnosis of the malignancy.
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