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Cells and Their Environment
Chapter 4
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4.1 Passive Transport Part 1: Diffusion
Remember: Living things need to maintain homeostasis (responds to external conditions to maintain a stable internal condition) One way to do this is controlling the movement of things in and out of a cell
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Passive Transport Requires no energy
Diffusion is the movement of a substance from an area of high concentration to an area of low concentration by random movement A difference in concentration of a substance across a space is a “concentration gradient”
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Equilibrium is where the concentration is equal
Since the interior of a cell is different from its external environment substances move back and forth through the cell membrane The membranes lipid interior repels ions and polar molecules. WHY!!
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The diffusion of small or nonpolar particles across the cell membrane is the simplest form of passive transport
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Part 2: Osmosis The diffusion of water through a selectively permeable membrane Water moves down the concentration gradient When something is dissolved in water its molecules are attracted to the water molecules This causes a difference between “free water” and “non-free water” This causes the concentration gradient
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Hypertonic Solution Fluid outside has higher concentration of dissolved substances than fluid inside the cell Causes water to diffuse out of cell Cell shrinks
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Hypotonic Solution Fluid inside has higher concentration of dissolved substances than fluid outside cell Causes water to diffuse into cell Cell swells
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Isotonic Solution Fluid inside has same concentration of dissolved substances as fluid outside cell Causes no net water movement Water moves in and out at equal rates Equilibrium – no change in size
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How does this affect you?
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Cells have ways of dealing with swelling or shrinking
Cell walls keep cells from expanding too much Some unicellular eukaryotes have contractile vacuoles to collect excess water and force it out Animal cells remove dissolved particles to stop osmosis
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Part 3: Crossing the Cell Membrane
Transport Proteins called channels help polar substances cross cell membrane Each only allows 1 kind of substance through
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Diffusion through ion channels
Many ions are important for cell functions Important in heartbeat and nerve cells Ion channels are transport proteins with a polar pore so ions can pass through membrane Ions pass without touching nonpolar lipid tails
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No use of energy so Passive Transport
Ion channel gates allow some to open and close in response with stimuli Stretching of cell membrane Change in electrical charge Binding of specific molecules to channel No use of energy so Passive Transport
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Electrical Charge and Ion Transport
Positive or negative charge affects movement of charged particle across membrane Inside of cells are negatively charged compared to outside This causes positively charged particles to want to move in and the negative ones to want to move out (trying to reach equilibrium)
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Facilitated Diffusion
Carrier Proteins (a kind of transport protein) bind to a specific substance and carry it across membrane and release it Still moving down concentration gradient Carrier protein attaches to substance on one side Shape of protein changes bringing substance through Substance protected from lipids and is released and protein goes back to original shape Passive Transport
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4.2 Active Transport Part 1: Movement Against a Concentration Gradient
When concentration is higher inside than outside, cells must move things against gradient from low to high Called Active Transport Requires energy, usually ATP
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Sometimes uses carrier proteins
Since moving against gradient, called membrane pumps
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Sodium-Potassium Pump
3 sodium ions, Na+, out and two potassium ions, K+, in Active transport of both Sodium higher outside and potassium higher inside Energy supplied by ATP
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How Does ATP Work? ATP is Adenosine TriPhosphate
3 phosphate groups attached to the sugar Ribose and the base, Adenine
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Phosphates are negative so they repel each other and are unstable
A little energy in causes one phosphate to break off and more energy is given off This causes ATP to become ADP, Adenosine DiPhosphate Cell uses energy given off from this reaction
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Sodium-Potassium Pump
3 Na+ inside bind to pump and a phosphate group leaves ATP and binds to pump Pump changes shape and 3 Na+ are released outside 2 K+ bind to pump and are transported across 2 K+ and P from ATP is released inside cell
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Allows K+ to be brought in
Pump prevents Na+ from building up in cell since it constantly flows in due to diffusion This build up would cause water to flow in due to osmosis, causing the cell to swell or burst Allows K+ to be brought in Many cells use Na+ pump to bring in things they need
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Part 2: Movement in Vesicles
Some things are too large to use carrier proteins Vesicles used instead
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Endocytosis when moved in
Cell membrane forms a pouch around a substance, closes up and pinches off forming vesicle Vesicle may fuse with lysosomes or other organelles
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Exocytosis when moved out
Vesicles inside fuse with membrane and release contents Used for proteins modified by Golgi apparatus Nerve cells and gland cells release proteins
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