Apodization without loss of resolution in the 3-D reconstruction of icosohedral virions

Peter D. Lauren; Michael B. Merickel; William W. Newcomb; Jay C. Brown

doi:10.1117/12.18918

1 July 1990 Apodization without loss of resolution in the 3-D reconstruction of icosohedral virions

Peter D. Lauren, Michael B. Merickel, William W. Newcomb, Jay C. Brown

Proceedings Volume 1233, Medical Imaging IV: Image Processing; (1990) https://doi.org/10.1117/12.18918
Event: Medical Imaging '90, 1990, Newport Beach, CA, United States

Abstract

ICOS is a modular software system for the 3-dimensional reconstruction of icosohedral particles from 2-dimensional projections. Noise in the resulting reconstructions is due to many sources including ringing artifact due to finite sampling of Fourier transforms and is comparable with many of the desired components representing particulate structure. This paper describes a method for reducing ringing artifact without significant loss of resolution. Application of a Blackman window to the original Fourier transforms significantly improves the signal to noise ratio by suppressing ringing artifact but reduces resolution in the fmal reconstruction. Taking a voxel by voxel minimum of the filtered and unfiltered 3-dimensional reconstruction restores the original resolution while retaining the enhanced SNR. 2.

Citation Download Citation

Peter D. Lauren, Michael B. Merickel, William W. Newcomb, and Jay C. Brown "Apodization without loss of resolution in the 3-D reconstruction of icosohedral virions", Proc. SPIE 1233, Medical Imaging IV: Image Processing, (1 July 1990); https://doi.org/10.1117/12.18918

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