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JRM Vol.20 No.1 pp. 38-46
doi: 10.20965/jrm.2008.p0038
(2008)

Paper:

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Development of the MOSFET Type Enzyme Biosensor Using GOx and ChOx

Katsutoshi Ooe*, Yasutaro Hamamoto**, Toshifumi Kadokawa**,
and Yoshiaki Hirano**

*Dept. of Micro System Technology, Faculty of Science and Engineering, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga 525-8577, Japan

**Dept. of Applied Chemistry, Faculty of Engineering, Osaka Institute of Technology, 5-16-1 Omiya, Asahi-ku, Osaka 535-8585, Japan

Received:
September 5, 2006
Accepted:
March 30, 2007
Published:
February 20, 2008
Creative Commons License
Keywords:
biosensor, MOSFET, glucose oxidase, cholesterol oxidase, health monitoring system
Abstract
As the population ages, the management of health is one of the important problems. Before now, we developed the glucose sensor for using at Health Monitoring System (HMS). The whole blood contains the manifold health index markers, and it is very important to measure them. Glucose sensor specifically detects the glucose of the blood, and it monitors the glucose concentration. Our glucose sensor had ‘separated Au electrode’ which immobilized glucose oxidase (GOx). By utilizing this style, it becomes possible that the sensor part is easily miniaturized. In our previous work, GOx was immobilized onto electrode by using of SAMs (Self-Assembled Monolayor) method, and the sensor using this working electrode detected the glucose concentration of glucose aqueous solution. In this paper, glucose sensor which immobilized GOx using the cross-link method was produced. And we carried out operation confirmation of produced glucose sensor using diluted human serum and whole blood. In addition, the cholesterol sensor which immobilized cholesterol oxidase (ChOx) onto separated Au electrode by cross-link method was produced. The immobilization of the ChOx was evaluated from the spectra of ESCA (Electron Spectroscopy for Chemical Analysis), and the performance as a sensor was evaluated.
Cite this article as:
K. Ooe, Y. Hamamoto, T. Kadokawa, and Y. Hirano, “Development of the MOSFET Type Enzyme Biosensor Using GOx and ChOx,” J. Robot. Mechatron., Vol.20 No.1, pp. 38-46, 2008.
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