Aminoallyl-UTP
Aminoallyl-UTP is used for Nucleic Acid Sequence Based Amplification (NASBA), Microarray based hybridization detection, Eberwine mRNA amplification and RNA-labeling. It is enzymatically incorporated into RNA during the NASBA reaction and Eberwine mRNA amplification. The resulting amine-functionalized RNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microarray based hybridization detection.
References:
[1] Scheler et al. (2009) Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications. BMC Biotechnol. 9:45.
[2] Hoen et al. (2003) Fluorescent labelling of cRNA for microarray applications. Nucleic Acids Res. 31 (5):e20.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 536.19(Anion) |
| Cas No. | 112131-73-4 |
| Formula | C12H17N3O15P3 (Anion) |
| Synonyms | 5-(3-Aminoallyl)-uridine-5'-triphosphate,aa-utp,aa utp,5-AA-UTP,5-Aminoallyluridine-5'-Triphosphate |
| Solubility | Soluble in sterile water |
| Chemical Name | ((2R,3S,4R,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C(N1)N([C@@H]2O[C@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[C@@H](O)[C@H]2O)C=C(/C=C/CN)C1=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
