Render Target: STATIC
Render Timestamp: 2024-12-23T11:35:26.344Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-26 17:01:08.955
Product last modified at: 2024-11-15T23:15:07.529Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

AQP5 Antibody #59558

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 22
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AQP5 Antibody recognizes endogenous levels of total AQP5 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other aquaporin family members.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu262 of human AQP5 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Aquaporin 5 (AQP5) is a plasma membrane water channel whose overexpression in human cancers, such as breast, pancreatic, and colon cancers, has been associated with poor prognosis and metastasis. In colorectal cancer cells, AQP5 has been shown to regulate migration and invasion, as well as expression of epithelial-mesenchymal transition (EMT) factors. Knockdown of AQP5 inhibits the Wnt/β-Catenin pathway (1). Localization of AQP5 is altered in gastric cancer, and AQP5 is a marker for pyloric stem cells (2). In hepatocellular carcinoma (HCC), downregulation of AQP5 inhibits metastasis and EMT through inactivation of the NF-κB signaling pathway, and therefore, AQP5 is a potential therapeutic target for HCC (3). Phosphorylation of AQP5 at Ser156, possibly by PKA, activates the Ras signaling pathway in NIH/3T3 cells (4). AQP5 alters cell shape and actin dynamics without inducing EMT in normal epithelial Madin-Darby canine kidney (MDCK) cells, and this effect likely requires phosphorylation of AQP5 at Ser156 (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.