It has been proposed that a drive for efficient communication shapes systems of semantic categories across languages. Recent work in this vein has increasingly emphasized communicative need: how often a particular object or idea will need to be referenced. Many studies assume for simplicity that the distribution of need across referents is the same for different cultures, and that this need distribution can be reliably inferred from corpora. In contrast, we elicited culture-specific estimates of communicative need from native speakers of English and Chinese. We compared those need distributions to each other and to a corpus-based need distribution, and we assessed the efficiency of the English and Chinese naming systems for the semantic domain of household containers under different need distributions. Our results suggest that languages reflect culture-specific need patterns, and that subjective estimates are sometimes superior to corpus data as a measure of need.

Is “cow” more closely related to “grass” or to “chicken”? Speakers of different languages judge similarity in this context differently, but why? One possibility is that cultures covarying with these languages induce differences in conceptualizations of similarity. Specifically, East Asian cultures may promote reasoning about thematic similarity, by which cow and grass are more related, whereasWestern cultures may bias judgments toward taxonomic relations, like cow and chicken. We measure similarity judgments across the US, China, and Vietnam and replicate US-China differences, but do not find that responding in Vietnam patterns with China. Instead, similarity judgments in Vietnam are intermediate between the US and China. We also show that word embedding models (fastText models for each language) are related to judgments within each country, suggesting a possible alternative interpretation of cross cultural differences. Perhaps notions of similarity are similar across contexts, but the statistics of the linguistic environment vary.

People perceive, think, and act in a multitude of different ways across cultures, and there is an extensive history of research documenting these differences. At the heart of much of this work is a contrast between Western and East Asian cultures that has inspired important efforts to document human psychology in populations outside of the WEIRD (Western, Educated, Industrial, Rich, Democratic) demographic, which is much overrepresented within psychological research (Henrich, Heine, & Norenzayan, 2010). Recent recommendations for measuring cultural distance (Muthukrishna et al., 2020) profile the US and China as focal points for cultural comparisons, but define cultural distance using explicit self-report measures. Here, we evaluate cross-cultural differences between the US and China using implicit and experimental measures. We attempt to reproduce and test extensions of prior work demonstrating cross-cultural differences in reasoning, vision, and social cognition with convenience snowball samples of university students. Few of these differences appeared in our sample.

Understanding the circumstances under which insect herbivores will adopt a novel host plant is a longstanding question in basic and applied ecology. While geographic variation in host use can arise through differences in both herbivore preference and plant characteristics, there is a tendency to attribute geographic variation in host use to regional differences in herbivore preference alone. This is especially true for herbivores specialized to one or a few plant species. We compared how geographic variation in herbivore preference and host plant origin shape regional differences in host plant use by the specialized herbivore, Euphydryas phaeton. In parts of its range, E. phaeton uses only a native host, Chelone glabra, while in others, it also uses an introduced host, Plantago lanceolata. We offered female butterflies from each region the non-native host plant sourced from both regions and compared their oviposition behavior. The non-native host was almost universally rejected by butterflies in the region where only the native plant is used. In the region where butterflies use both hosts, females accepted non-native plants from their natal region twice as often as non-native plants from the other region where they are not used. Acceptance differed substantially among individual butterflies within regions but not among plants within regions. Thus, both individual preference and regional differences in both the insect and non-native host contributed to the geographic variation in different ways. These results highlight that, in addition to herbivore preference, regional differences in perceived plant suitability may be an important driver of diet breadth.

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo . Single-photon avalanche photodiode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster Resonant Energy Transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated-ICCD camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster resonant energy transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated intensified charge-coupled device (ICCD) camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.

Background

Cardiac dysfunction in failing hearts of human patients and animal models is associated with both microtubule densification and transverse-tubule (T-tubule) remodeling. Our objective was to investigate whether microtubule densification contributes to T-tubule remodeling and excitation-contraction coupling dysfunction in heart disease.

Methods and results

In a mouse model of pressure overload-induced cardiomyopathy by transaortic banding, colchicine, a microtubule depolymerizer, significantly ameliorated T-tubule remodeling and cardiac dysfunction. In cultured cardiomyocytes, microtubule depolymerization with nocodazole or colchicine profoundly attenuated T-tubule impairment, whereas microtubule polymerization/stabilization with taxol accelerated T-tubule remodeling. In situ immunofluorescence of heart tissue sections demonstrated significant disorganization of junctophilin-2 (JP2), a protein that bridges the T-tubule and sarcoplasmic reticulum membranes, in transaortic banded hearts as well as in human failing hearts, whereas colchicine injection significantly preserved the distribution of JP2 in transaortic banded hearts. In isolated mouse cardiomyocytes, prolonged culture or treatment with taxol resulted in pronounced redistribution of JP2 from T-tubules to the peripheral plasma membrane, without changing total JP2 expression. Nocodazole treatment antagonized JP2 redistribution. Moreover, overexpression of a dominant-negative mutant of kinesin 1, a microtubule motor protein responsible for anterograde trafficking of proteins, protected against JP2 redistribution and T-tubule remodeling in culture. Finally, nocodazole treatment improved Ca(2+) handling in cultured myocytes by increasing the amplitude of Ca(2+) transients and reducing the frequency of Ca(2+) sparks.

Conclusion

Our data identify a mechanistic link between microtubule densification and T-tubule remodeling and reveal microtubule-mediated JP2 redistribution as a novel mechanism for T-tubule disruption, loss of excitation-contraction coupling, and heart failure.