On this paper we show records of Pleistocene fauna from the archaeological site of PayPaso 1, located near of the Quarai River. On this site we recovered two extinct species, Equus sp. (ancient horse) e Glyptodon sp. (giant armadillo), direct associated with lithic artifacts. Our results indicate that these extinct mammals lived in the beginning of the Holocene (9,600 – 9,100 years 14C BP), based on nine 14C age results obtained by AMS (Accelerator Mass Spectrometry) measurements. In this work, these results are compared with others in South America. Human adaptation, lithic technology,Pleistocene fauna extinction and climate change at the transition between leistocene-Holocene are also discussed.