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    THE LOBSTER:: a Model for Teaching Neurophysiological Concepts
    THE LOBSTER:: a Model for Teaching Neurophysiological Concepts
    THE LOBSTER:: a Model for Teaching Neurophysiological Concepts
    Ebook63 pages34 minutes

    THE LOBSTER:: a Model for Teaching Neurophysiological Concepts

    By Kaitlyn E. Brock, Robin L. Cooper and Alaina C. Taul

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    About this ebook

    Similar to the sciatic nerve, lobster nerves are useful tools for teaching conduction velocity, examining pharmacological actions, addressing recruitment of neurons, obtaining compound action potentials, and covering the principles of refractory periods.

    This book shows how to obtain lobster nerve preparations and to record synaptic transmission at neuromuscular junctions and measure sensory proprioception—all within a single animal.
    LanguageEnglish
    PublisherAzalea Art Press
    Release dateMay 12, 2024
    ISBN9781943471850
    THE LOBSTER:: a Model for Teaching Neurophysiological Concepts

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      Book preview

      THE LOBSTER: - Kaitlyn E. Brock

      a Model for Teaching

      Neurophysiological Concepts

      Kaitlyn E. Brock

      and Robin L. Cooper

      COPYRIGHT:

      Azalea Art Press

      Sonoma . California

      © Kaitlyn E. Brock, Robin L. Cooper, 2024.

      All Rights Reserved.

      ISBN: 978-1-943471-85-0

      A close-up of a bar code Description automatically generated

      This work is licensed under a Creative Commons Share Alike agreement that material from this book may be used as long as attribution is given to the source of the material.

      A group of black symbols Description automatically generated

      Cover illustration by:

      Kaitlyn Brock and Alaina C. Taul

      CONTENTS:

      Abstract

      Terms

      Preface

      Introduction

      Part I

      Methods

      Part II

      Dissections and Recordings

      in the Various Preparations

      Part III

      Discussion

      References

      Funding

      Acknowledgments

      About the Authors

      Kaitlyn E. Brock

      Robin L. Cooper

      Contact|Book Orders

      A cartoon of a purple object Description automatically generated

      Abstract

      The frog sciatic nerve remains a common educational model for animal and neurophysiology laboratories. It is used to teach principles of nerve conduction and related biophysical properties. However, animal care regulations and maintenance required for vertebrates pose some challenges. An alternative preparation used to teach the same principles as with the sciatic nerve may be beneficial. The lobster preparation allows for the extraction of 10 to 20 nerves from a single animal. Lobsters are invertebrates, meaning there are fewer regulatory restrictions. They are relatively easy to house. This model would reduce the number of animals euthanized as a laboratory-based model for neurophysiology. Similar to the sciatic nerve, the lobster nerves can be used for teaching about conduction velocity, examining pharmacological actions, addressing recruitment of neurons, obtaining compound action potentials, and covering the principles of refractory periods. Lobster nerve preparations are relatively hardy and viable for hours at room temperature. They can be maintained for days if stored in a refrigerator.

      This report explains how to obtain lobster nerve preparations and record from them for educational purposes. In addition, it demonstrates the recording of synaptic transmission at neuromuscular junctions and measures sensory proprioception all within a single animal.

      Terms

      Compound action potential (CAP) - an extracellular recording of a summated electrical event from a nerve in response of a population of neurons.

      Spike - Extracellular recording of an action potential from a nerve.

      EJP - excitatory junction potential recorded in a muscle.

      IJP - inhibitory junction potential recorded in a muscle.

      Preface

      This text is presented to provide this protocol for teaching neurophysiological concepts using the lobster as a model to anyone in the world. This resource will be open and free under a Creative Commons Share Alike (CC BY-SA) license, or at a low cost to download. This is a test run on inexpensive publishing of teaching protocols for our research group. If it proves to be of interest, additional educational protocols will be forthcoming.

      This book

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