Version 1
: Received: 12 September 2019 / Approved: 16 September 2019 / Online: 16 September 2019 (16:41:43 CEST)
How to cite:
Pareja-Tobes, E.; Tobes, R. ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling. Preprints2019, 2019090170. https://doi.org/10.20944/preprints201909.0170.v1
Pareja-Tobes, E.; Tobes, R. ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling. Preprints 2019, 2019090170. https://doi.org/10.20944/preprints201909.0170.v1
Pareja-Tobes, E.; Tobes, R. ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling. Preprints2019, 2019090170. https://doi.org/10.20944/preprints201909.0170.v1
APA Style
Pareja-Tobes, E., & Tobes, R. (2019). ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling. Preprints. https://doi.org/10.20944/preprints201909.0170.v1
Chicago/Turabian Style
Pareja-Tobes, E. and Raquel Tobes. 2019 "ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling" Preprints. https://doi.org/10.20944/preprints201909.0170.v1
Abstract
Here we describe ARSA-16S, a tool and accompanying reference database for the analysis of bacterial 16S amplicons. Among other features, ARSA-16S is based on a new model, approach, and algorithm for sequence-level assignment of reads understood as probability distributions, assigns reads individually, and is designed with non-overlapping amplicons covering two non-contiguous regions. A new set of primers for the amplification and sequencing of the V4 and V6 regions is also provided.
Keywords
16S analysis, metagenomics, amplicons, bioinformatics, DNA sequencing
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.