Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Aggregation-Induced Emission Luminogens (AIEgens):A New Possibility for Efficient Visualization of RNA in Plants

Version 1 : Received: 21 November 2023 / Approved: 22 November 2023 / Online: 22 November 2023 (10:30:11 CET)

A peer-reviewed article of this Preprint also exists.

Yang, Z.-C.; Zhao, L.-X.; Sang, Y.-Q.; Huang, X.; Lin, X.-C.; Yu, Z.-M. Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants. Plants 2024, 13, 743. Yang, Z.-C.; Zhao, L.-X.; Sang, Y.-Q.; Huang, X.; Lin, X.-C.; Yu, Z.-M. Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants. Plants 2024, 13, 743.

Abstract

RNA, as an important substance for regulating biological growth and development, has significant implications for visualization research. However, many spontaneously fluorescent substances in plants greatly interfere with the effectiveness of plant bioimaging. Aggregetion- induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. We have found that AIEgens have great potential as RNA fluorescent probes for efficient imaging in plants. In this review, we first introduce several common RNA labeling forms and point out their pros and cons. We briefly describe the development of AIEgens and the AIE mechanism, and then present various practical applications of AIEgens, including detailed examples of their use as biological markers. To further promote the application of AIE in the field of RNA, we suggest the use of AIEgens to modify target RNA via techniques such as click chemistry or CRISPR/Cas, to achieve RNA visualization in plants. highly possible to modify target RNA with AIEgens in vivo for RNA visualization.

Keywords

aggregation-induced emission (AIE); RNA labeling; RNA aptamer; GFP; CRISPR/Cas; click chemistry

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.