Version 1
: Received: 30 September 2024 / Approved: 1 October 2024 / Online: 1 October 2024 (04:55:01 CEST)
How to cite:
Du, B.; Chen, T.; Huang, A.; Chen, H.; Liu, W. Portable Detection of Copper Ion Using Personal Glucose Meter. Preprints2024, 2024100011. https://doi.org/10.20944/preprints202410.0011.v1
Du, B.; Chen, T.; Huang, A.; Chen, H.; Liu, W. Portable Detection of Copper Ion Using Personal Glucose Meter. Preprints 2024, 2024100011. https://doi.org/10.20944/preprints202410.0011.v1
Du, B.; Chen, T.; Huang, A.; Chen, H.; Liu, W. Portable Detection of Copper Ion Using Personal Glucose Meter. Preprints2024, 2024100011. https://doi.org/10.20944/preprints202410.0011.v1
APA Style
Du, B., Chen, T., Huang, A., Chen, H., & Liu, W. (2024). Portable Detection of Copper Ion Using Personal Glucose Meter. Preprints. https://doi.org/10.20944/preprints202410.0011.v1
Chicago/Turabian Style
Du, B., Hanjun Chen and Wei Liu. 2024 "Portable Detection of Copper Ion Using Personal Glucose Meter" Preprints. https://doi.org/10.20944/preprints202410.0011.v1
Abstract
A simple and sensitive method for Cu²⁺ detection was developed using the Cu⁺-catalyzed alkyne-azide cycloaddition reaction, Fe3O4 magnetic nanoparticles (MNPs) as the reaction platform, and a portable blood glucose meter (PGM) as the detection method. Gold nanoparticles (AuNPs) were labeled with glucose oxidase (GOx) and alkyne-functionalized and terminally thiolated ssDNA (C1). In the presence of Cu²⁺ and ascorbate, the functionalized AuNPs were captured onto MNPs modified with azide-functionalized ssDNA (C2) via the Cu⁺-catalyzed alkyne-azide cycloaddition reaction. GOx on the AuNPs surface oxidized glucose (Glu) into gluconic acid and H2O2, reducing the Glu content in the reaction solution, which was quantitatively detected by the PGM. Under optimal conditions, the PGM response of the system showed a good linear relationship with the logarithm of Cu²⁺ concentration in the range of 0.05 to 10.00 μmol/L, with a detection limit of 0.03 μmol/L (3σ). In actual tap water samples, the spiked recovery rate of Cu²⁺ was between 92.30% and 113.33%, and the relative standard deviation was between 0.14% and 0.34%, meeting the detection requirements for Cu²⁺ in real water samples.
Keywords
Personal glucose meter; Copper ion; Fe3O4 magnetic nanoparticles; Alkyne-azide cycloaddition reaction
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
