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    Pet200dtopo Mcs

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    api-19771915
    This document contains the sequence of a DNA plasmid. It includes elements like a T7 promoter, lac operator, ribosome binding site, and sequences encoding an epitope tag and polyhistidine region. The plasmid allows expression of a protein with an N-terminal epitope tag and polyhistidine region for purification and detection.

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    Pet200dtopo Mcs

    Uploaded by

    api-19771915
    16 views1 page
    This document contains the sequence of a DNA plasmid. It includes elements like a T7 promoter, lac operator, ribosome binding site, and sequences encoding an epitope tag and polyhistidine region. The plasmid allows expression of a protein with an N-terminal epitope tag and polyhistidine region for purification and detection.

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    pet200dtopo_mcs

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    This document contains the sequence of a DNA plasmid. It includes elements like a T7 promoter, lac operator, ribosome binding site, and sequences encoding an epitope tag and polyhistidine region. The plasmid allows expression of a protein with an N-terminal epitope tag and polyhistidine region for purification and detection.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    16 views1 page

    Pet200dtopo Mcs

    Uploaded by

    api-19771915
    This document contains the sequence of a DNA plasmid. It includes elements like a T7 promoter, lac operator, ribosome binding site, and sequences encoding an epitope tag and polyhistidine region. The plasmid allows expression of a protein with an N-terminal epitope tag and polyhistidine region for purification and detection.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC

    T7 promoter/priming site
    T7 promoter lac operator

    201 CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA

    RBS Nde I Polyhistidine region Nhe I


    281 AGAAGGAGAT ATACAT ATG CGG GGT TCT CAT CAT CAT CAT CAT CAT GGT ATG GCT AGC ATG ACT GGT GGA
    Met Arg Gly Ser His His His His His His Gly Met Ala Ser Met Thr Gly Gly
    XpressTM epitope
    351 CAG CAA ATG GGT CGG GAT CTG TAC GAC GAT GAC GAT AAG GAT CAT CCC TT C ACC ... ... AAGGGC
    GGG AAG TGG ... ...

    G
    Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp His Pro Phe Thr

    TG
    G
    EK recognition site
    EK cleavage site T7 reverse priming site
    411 GAGCTCAACG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA

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