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    The activated partial thromboplastin time (APTT) test measures the intrinsic clotting pathway by incubating plasma with a contact activator like kaolin and phospholipids. It is sensitive to deficiencies in coagulation factors VIII, IX, XI, XII, prekallikrein, high molecular weight kininogen, and to the presence of heparin. The test involves incubating plasma with APTT reagent for 3 minutes, then adding calcium chloride to initiate clotting. The time to clot formation is measured and compared to a normal range of 30-40 seconds. Prolonged APTT can indicate coagulation factor deficiencies, liver disease, disseminated intravascular coagulation, he

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    The activated partial thromboplastin time (APTT) test measures the intrinsic clotting pathway by incubating plasma with a contact activator like kaolin and phospholipids. It is sensitive to deficiencies in coagulation factors VIII, IX, XI, XII, prekallikrein, high molecular weight kininogen, and to the presence of heparin. The test involves incubating plasma with APTT reagent for 3 minutes, then adding calcium chloride to initiate clotting. The time to clot formation is measured and compared to a normal range of 30-40 seconds. Prolonged APTT can indicate coagulation factor deficiencies, liver disease, disseminated intravascular coagulation, he

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    The activated partial thromboplastin time (APTT) test measures the intrinsic clotting pathway by incubating plasma with a contact activator like kaolin and phospholipids. It is sensitive to deficiencies in coagulation factors VIII, IX, XI, XII, prekallikrein, high molecular weight kininogen, and to the presence of heparin. The test involves incubating plasma with APTT reagent for 3 minutes, then adding calcium chloride to initiate clotting. The time to clot formation is measured and compared to a normal range of 30-40 seconds. Prolonged APTT can indicate coagulation factor deficiencies, liver disease, disseminated intravascular coagulation, he

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    © All Rights Reserved
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    Download as doc, pdf, or txt
    26 views2 pages

    Format Content

    Uploaded by

    Peter Limjoco David
    The activated partial thromboplastin time (APTT) test measures the intrinsic clotting pathway by incubating plasma with a contact activator like kaolin and phospholipids. It is sensitive to deficiencies in coagulation factors VIII, IX, XI, XII, prekallikrein, high molecular weight kininogen, and to the presence of heparin. The test involves incubating plasma with APTT reagent for 3 minutes, then adding calcium chloride to initiate clotting. The time to clot formation is measured and compared to a normal range of 30-40 seconds. Prolonged APTT can indicate coagulation factor deficiencies, liver disease, disseminated intravascular coagulation, he

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    UNIVERSITY OF SANTO TOMAS

    FACULTY OF PHARMACY
    DEPARTMENT OF MEDICAL TECHNOLOGY
    Principles and Strategies of Teaching in Health Education
    TEACHING DEMONSTRATION
    ACTIVATED PARTIAL THROMBOPLASTIN TIME
    I.

    OVERVIEW
    This test is also known as partial thromboplastin time with Kaolin (PTTK), and the Kaolin

    Cephalin Clotting Time (KCCT).


    II.

    PRINCIPLE
    The test measures the clotting time of plasma after the activation of contact factors but

    without added in tissue extract (thromboplastin) and so indicates the overall efficiency of the
    intrinsic clotting system. To standardize the activation of the contact factors, the plasma is first
    pre-incubated with Kaolin. A standardized phospholipid is provided to allow the test to be
    performed in platelet poor plasma. The test not only depends on the contact factors and VIII and
    IX, but also on the reactions with factors X, V, Prothrombin and Fibrinogen. It is also sensitive to
    the presence of circulating anticoagulants (inhibitors) and heparin.
    III.

    REAGENTS
    A.
    Patient and control citrated plasma (PPP).
    B.
    APTT reagent, (phospholipid + Kaolin)
    C.
    CaCl2 0.025 mol/l.

    IV.

    METHOD
    A.
    Keep the reagent in 37 OC for 10 min
    B.
    Add 0.1 ml of PTT reagent.
    C.
    Add 0.1 ml of plasma and incubate the mixture for 3 min at 37 OC
    D.
    Add 0.1 ml of pre-warmed CaCl2 and mix the contents of the tube.
    E.
    Start the stop-watch and record the end point.
    F.
    Carry out the test in duplicate on the patient's and control plasma.

    V.

    NORMAL RANGE
    The normal range for this test is 30-40 seconds.

    VI.

    INTERPRETATION:
    The common causes of prolonged PTTK in acute hemostatic failure are:
    A.
    The administration of heparin or contaminated with heparin.
    B.
    Liver diseases.
    C.
    Disseminated Intravascular Coagulation.
    D.
    Coagulation factor deficiency or deficiency, hemophilia A, hemophilia B, and
    factor IX and factor XII, PK, HMWK, V, Prothrombin or fibrinogen deficiency.

    UNIVERSITY OF SANTO TOMAS


    FACULTY OF PHARMACY
    DEPARTMENT OF MEDICAL TECHNOLOGY
    Principles and Strategies of Teaching in Health Education
    TEACHING DEMONSTRATION
    E.

    Massive transfusion with stored blood.

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