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    Microbilology - Unknown Lab Report

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    AldenSchulte
    A college level microbiology lab report on finding two unknown microorganisms. This is free to use as an example if someone needed a good look at a completed unknown microbiology lab report.

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    © All Rights Reserved
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    Microbilology - Unknown Lab Report

    Uploaded by

    AldenSchulte
    186 views2 pages
    A college level microbiology lab report on finding two unknown microorganisms. This is free to use as an example if someone needed a good look at a completed unknown microbiology lab report.

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    © © All Rights Reserved

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    A college level microbiology lab report on finding two unknown microorganisms. This is free to use as an example if someone needed a good look at a completed unknown microbiology lab report.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    186 views2 pages

    Microbilology - Unknown Lab Report

    Uploaded by

    AldenSchulte
    A college level microbiology lab report on finding two unknown microorganisms. This is free to use as an example if someone needed a good look at a completed unknown microbiology lab report.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
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    Alden Schulte

    Microbiology
    Unknown Report
    Dr. Anderson ASUB 2013

    Alden Schulte
    Microbiology
    Lab Unknown

    When I received my unknown mixed culture I decided the first thing I needed to do was
    separate the bacteria in the culture. First I performed a streak plate to isolate colonies of the
    different bacteria. While I was waiting for my streak plate to incubate, I carried out a gram stain
    in order to get a better look at what I was dealing with. Looking at the gram stained mixed
    culture I could clearly see purple cocci shapes and pink bacillus shapes. When my streak plate
    was finished incubating I could clearly distinguish between the two bacteria from the physical
    variations of the colonies they formed. At this point I gram stained both colonies separately to
    make sure I had the same results as before, I did. Gram positive cocci and gram negative
    bacillus were plainly visible. Now that I had separate colonies I inoculated the two colonies on
    different slant agars, as to obtain a pure culture for testing each. After my slants were done
    incubating I once again gram stained both sets of bacteria, this time to ensure I had successfully
    obtained pure cultures, which I had. Now that I had my cultures, I decided it best to test both
    bacteria for their preferred oxygen usage, both of which were found to be fermentative. After
    that I realized there was quite a bit of difference in the bacteria overall on the various sugar
    tests. So I decided that to get more valuable information I would run the glucose, lactose,
    sucrose, and mannitol tests on both cultures.
    In organism 1 (gram + cocci) all of the sugar tests results were positive. After this I
    decided that to narrow down the few organisms I had left I needed to run the catalase and
    coagulase tests, the results of which were both positive. After these two tests I had ended up
    with one organism that matched all the data I had collected. My gram + cocci were
    Staphylococcus aureas.
    In organism 2 (gram - bacillus) all of the sugar tests results were positive. The lactose
    test made a huge difference in my search, taking me from 13 organisms to 4. I decided to
    perform the methyl red and VP tests to take some possibilities away from the equation. My
    methyl red test turn out positive, while my VP test showed negative. After all these tests I had
    one organism that matched all the data I had collected. My gram - bacillus was Escherichia coli.

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