Cerebrospinal Fluid
Cerebrospinal Fluid
Cerebrospinal Fluid
CSF Formation
CSF between pia and arachnoid
maters
subarachnoid space.
CSF produced by ventricular
capillary blood vessels
choroid plexuses
CSF Volumes and Function
Total CSF volumes:
Adult 85 - 150 ml
Neonate 10 - 60 ml
Adult Rate of Formation 500 ml/day
Turn over = 20 mL/hour
PHYSIOLOGY
1. To supply nutrients to the nervous system
2. To remove metabolic wastes
3. To produce a mechanical barrier to cushion the brain
and spinal cord against trauma.
CSF Collection Indications
Meningeal
infection
Subarachnoi
Fever of
d
hemorrhage
Unknown Malignancy
Origin
Neurological
disease
CSF
Cells counting:
Viscosity Macroscopic Total cellular count
WBCs and differential
Turbidity (Gross)
Performed on a stained*
smear made from CSF.
It is recommended that
stained smears be made
even when the total cell
count is within normal
limits.
PMNs,
Lymphocytes
Monocyte
Lymphocytes
Phagocytized RBCs by
macrophages
Hematoidin Bodies in
macrophages
Graphic accessed URL http://www.geocities.com/jcprolla/481a.jpg, 2009.
CSF cytology. A. Nests of anaplastic tumor cells are seen with round to oval
eccentric nuclei, coarse chromatin, prominent nucleoli and moderate
Large lymphoid cells with dispersed chromatin, prominent homogeneous cytoplasm . B. Single giant malignant cells.
nucleoli, and scant cytoplasm in the CSF; observed in the one
case of typical MCL