Thermo Scientific Varioskan Flash combines fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. Unlimited wavelength selection gives ultimate flexibility for identifying the optimal measurement wavelength for any assay, now and in the future. In photometry, double monochromator design produces a very low stray light level ensuring excellent linearity.
Thermo Scientific Varioskan Flash combines fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. Unlimited wavelength selection gives ultimate flexibility for identifying the optimal measurement wavelength for any assay, now and in the future. In photometry, double monochromator design produces a very low stray light level ensuring excellent linearity.
Thermo Scientific Varioskan Flash combines fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. Unlimited wavelength selection gives ultimate flexibility for identifying the optimal measurement wavelength for any assay, now and in the future. In photometry, double monochromator design produces a very low stray light level ensuring excellent linearity.
Thermo Scientific Varioskan Flash combines fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. Unlimited wavelength selection gives ultimate flexibility for identifying the optimal measurement wavelength for any assay, now and in the future. In photometry, double monochromator design produces a very low stray light level ensuring excellent linearity.
Thermo Scientific Varioskan Flash together with SkanIt Software offers optimal performance for demanding research assays Thermo Scientific Varioskan Flash is a spectral scanning multimode reader, including fluorescence intensity, time-resolved fluorescence (TRF), photometric, and luminometric detection technologies. It offers optimal performance for demanding research assays with unlimited wavelength selection, up to three onboard dispensers, utmost optical performance and the advanced Thermo Scientific SkanIt Software.
Unlimited wavelength selection for assay optimization
The Thermo Scientic Varioskan Flash spectral scanning multimode reader combines uorescence intensity, time-resolved uorescence (TRF), photometric, and optional luminometric detection technologies. It provides unlimited wavelength selection, and thereby allows both spectral analysis and measurement at any single wavelength. This gives ultimate exibility for identifying the optimal measurement wavelength for any assay, now and in the future.
In photometry, double monochromator design produces a very low stray light level ensuring excellent linearity. Outstanding accuracy and precision are guaranteed with the perfect beam optics. Varioskan Flash has two dedicated detection optics for luminometry: Scanning optics that is excellent for studying and optimizing luminometric assay parameters, and the high sensitivity Varioskan LumiSens optics, specially designed for measurement of any luminometric assay with excellent sensitivity, including multilabel assays requiring wavelength selection.
reagents in any order or in any phase of the kinetic assay allows execution of sequential multistep assays, such as ATP and reporter gene applications. In addition, automated dispensing guarantees reproducible dispensing from day to day and from person to person.
Fluorescence intensity (top/bottom)
High-performance optical technology
High-quality performance is obtained with the optimized optics of Varioskan Flash. In uorometry, stray light transmission is minimized using quadruple monochromators. This ensures ultimate spectral quality that guarantees superior assay sensitivity. In addition, the quadruple monochromator optics offers excellent performance and exibility in measuring either single or multilabel assays over a wide concentration range. Furthermore, the bottom reading possibility enables the user to select the optimal reading position for uorometric measurement.
Onboard dispensers for exact follow-up of kinetic reactions
For easy and accurate reagent additions, Varioskan Flash can be equipped with up to three onboard dispensers. The dispenser facilitates the work and allows easier optimization of assays. The instrument supports simultaneous dispensing and measurement, thereby enabling follow-up of kinetic reactions directly from reaction onset. This capability is essential for ash type luminescence reactions, Ca2+ ux studies and other rapid kinetic applications. The ability to add Time-resolved uorescence (TRF)
Spectral scanning luminometry
High sensitivity luminometry (ash and glow)
UV/Vis photometry
Multilabel measurement (BRET, FRET, TR-FRET)
The utmost sensitivity and a wide dynamic range is achieved by Varioskan Flashs optical system with quadruple monochromators. Varioskan Flash supports various detection technologies.
Varioskan Flash capabilities provide versatility for assays
Assays requiring any measurement wavelength from low UV to near IR can be performed due to a wide spectral range of the Varioskan Flash. Photometric pathlength correction is ideal for direct measurement of DNA, RNA and proteins. Fluorometric UV measurement of uorescent amino acids is an efcient tool in labelfree assays for protein structure changes. Fluorescent cell based assays provide high-performance with Varioskan Flash due to selectable top or bottom reading, exibility in plate formats and a high-precision incubator. With TRF technology, Varioskan Flash offers great exibility for high-performance cellular assays without interference from biological background. Timeresolved uorescence resonance energy transfer (TR-FRET) assays are easily optimized using the TRF spectral scanning function of the Varioskan Flash. Additional TRF delay time optimization using automatic value calculation makes it straightforward to dene the best possible measurement parameters. The luminometric monochromator mode of Varioskan Flash offers a unique possibility to optimize measurement wavelengths in luminometric multilabel assays. Thereafter, assays can be performed with superior sensitivity with the normal or lter mode of the dedicated Varioskan LumiSens optics.
High-performance incubator for controlled assay conditions
To maintain optimal and constant reaction conditions, Varioskan Flash has a high-performance onboard incubator that ensures controlled assay conditions. It is ideal for cellular assays, enzyme assays and other applications where temperature control is essential.
Applications: Apoptosis assays Ca2+ flux assays Cell proliferation Cellular assays Cytotoxicity and ADMETox Direct DNA, RNA and protein quantitation ELISA/FIA/TRF-ELISA assays Enzyme kinetic studies Europium assays FRET assays TR-FRET assays BRET assays GPCR assays Ion channel assays Kinase assays Multilabel assays Reporter gene assays Signal transduction Tryptophan and tyrosine UV fluorescence
Specially designed for automation
Varioskan Flash has been designed for easy integration with automated systems. The robotic plate carrier of Varioskan Flash is specially designed for convenient robot arm access, allowing microplate gripping in both portrait and landscape congurations. Thermo Scientic SkanIt Software also has a special remote control interface for integration with automated systems and LIMS.
Logical assay setup using SkanIt Software
The powerful SkanIt Software allows easy assay optimization, exible data handling and convenient report formatting. The unique steplist feature makes assay setup highly visual and exible, and the workow logical and easy to follow. There are two editions of SkanIt Software available: a Research Edition for scientists working in life science research, and a Drug Discovery Edition offering features needed for compliance with the FDAs 21 CFR Part 11, for the drug discovery industry.
The Steplist in SkanIt Software makes assay setup visual and logical.
Endpoint, kinetic, spectral scanning with all detection technologies 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1) < 2 s/well, 400 500 nm, 1 ash, 2 nm steps 540 mm (W) x 580 mm (D) x 500 mm (H) [21.3 (W) x 22.8 (D) x 19.7 (H)]
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