Agilent 218 Purification System delivers isocratic flow or gradients with excellent precision and reproducibility. Easy-change pump heads allow you to span a broad flow range from as little as 0.01 mL / min to as much as 200 mL per minute. A unique dual-path detector cell expands your dynamic detection range from 0.005 mAU up to 80 absorbance units.
Agilent 218 Purification System delivers isocratic flow or gradients with excellent precision and reproducibility. Easy-change pump heads allow you to span a broad flow range from as little as 0.01 mL / min to as much as 200 mL per minute. A unique dual-path detector cell expands your dynamic detection range from 0.005 mAU up to 80 absorbance units.
Agilent 218 Purification System delivers isocratic flow or gradients with excellent precision and reproducibility. Easy-change pump heads allow you to span a broad flow range from as little as 0.01 mL / min to as much as 200 mL per minute. A unique dual-path detector cell expands your dynamic detection range from 0.005 mAU up to 80 absorbance units.
Agilent 218 Purification System delivers isocratic flow or gradients with excellent precision and reproducibility. Easy-change pump heads allow you to span a broad flow range from as little as 0.01 mL / min to as much as 200 mL per minute. A unique dual-path detector cell expands your dynamic detection range from 0.005 mAU up to 80 absorbance units.
Purify your way AGILENT 218 PURIFICATION SYSTEM PURIFY YOUR WAY WITH A FLEXIBLE SYSTEM WITHIN REACH OF YOUR BUDGET The Agilent 218 Purication System has the exibility to adapt to your purication needs as they change over time. Delivering isocratic ow or gradients with excellent precision and reproducibility, this system is a true workhorse for routine LC purication work. Whats more, its within reach of your budget. 2 Flexibility to Purify Your Way The Agilent 218 Purication System gives you the exibility to meet all your purication needs now and in the future. Easy-to-change pump heads allow you to span a broad ow range from as little as 0.01 mL/min to as much as 200 mL per minute. A unique dual- path detector cell expands your dynamic detection range from 0.005 mAU up to 80 absorbance units. Ideal for a huge number of laboratory-scale purication applications, the Agilent 218 Purication System can also handle your biological samples safely and securely. As purication needs change, its easy to extend the capabilities of the 218 system. Simply interchange the pump heads, tubing, mixers or other accessories to meet the new challenge. Its easy to upgrade to binary, ternary or quaternary gradient capability by adding additional pumps. Excellent ow precision and gradient accuracy for reproducible separations every time Automated sample Introduction for high- throughput purication of a large number of diverse samples Extended dynamic range for sensitive detection of high and low concentrations Intelligent real-time data processing for instantaneous and precise fraction collection Automated fraction collection for accurate, reliable and reproducible collection of your valuable samples Learn more: www.agilent.com/chem/purication 3 Precise and Reproducible Solvent Delivery Precise and reproducible solvent delivery is key to successful compound purication. The Agilent 218 Purication System has rugged and reliable solvent delivery modules, designed and manufactured to deliver highest ow precision and composition accuracy. The single-piston, rapid-ll design is capable of delivering solvent ow from 0.01 mL/min up to 200 mL/min, making the system versatile enough to meet all of your semi-preparative purication needs. Choose from isocratic or gradient solvent delivery modules that include pump heads and purge valves. Exceptional ow precision and compositional accuracy for reproducible separations every time Flow rate range from 0.01200 mL/min for maximum purication exibility Pressures up to 600 bar at 10 mL/min or 230 bar at 200 mL/min for semi-preparative separations using 1- or 2-inch inside diameter columns OpenLAB CDS for Complete Control Agilents OpenLAB Chromatography Data System (CDS) is powerful yet easy-to-use software that puts you in complete control of your processes. The softwares intuitive interface simplies method development as well as scale-up from analytical to preparative purication. The fraction preview function provides a simple graphical tool to transfer collection parameters from a test run to a preparative separation. Time, peak and mass-based fraction collection or any combination of these are available and can be triggered by any detector signal. Intelligent real-time data processing facilitates instantaneous and precise fraction collection. Full system control for standard purication functionality Peak-trigger options Graphical fraction analysis for data review Full support for 21 CFR Part 11 compliance Step gradient (upper trace) and overlay of ve injections demonstrate the precision and reproducibility of the Agilent 218 Purication Systems solvent delivery while running at 10 mL/min and equipped with 100 mL pump head. Agilent laboratory-scale purication systems are controlled through Agilent OpenLAB CDS a single software for analytical and preparative work. Absorbance [mAU] 0 0 Time [min] 10 20 30 40 50 60 50 100 150 200 250 300 Absorbance [mAU] 0 Time [min] 3 4 5 6 7 8 9 10 20 40 60 80 100 4 AGILENT 218 PURIFICATION SYSTEM CHOOSE A SYSTEM CONFIGURATION TO PURIFY YOUR WAY Whether you require instrumentation for general analytical purposes with occasional purication tasks, for automatic scale-up from method development using analytical and semi-preparative columns, or for dedicated semi-preparative work using 1- or 2-inch id columns, you can congure an Agilent 218 Purication System to meet your needs. And, whatever conguration you select, you can be condent the system will provide outstanding performance and reliability day-in, day-out. Isocratic System for Simple Separations For purifying samples by separation on normal phase, reversed- phase or chiral columns where gradient elution is not required, an isocratic conguration is a perfect solution. A wide range of interchangeable pump heads is available, giving you the exibility to deliver ow rates from 0.01 up to 200 mL per minute. A typical isocratic conguration comprises: Agilent 218 Solvent Delivery Module Agilent 325 Dual Wavelength UV-Visible Detector Agilent 440 Fraction Collector Agilent OpenLAB CDS Gradient System for Complex Separations For purication challenges requiring precise and reliable high-pressure gradients, congure an Agilent 218 Purication System with two solvent pumps. Addition of an autosampler facilitates automatic sample introduction for higher throughput. A typical gradient system comprises: Agilent 218 Solvent Delivery Modules Agilent 410 Autosampler Agilent 325 Dual Wavelength UV-Visible Detector Agilent 440 Fraction Collector Agilent OpenLAB CDS Isocratic system comprising pump, manual injection valve, detector and fraction collector. Gradient system comprising two pumps, autosampler, detector and fraction collector. 5 AGILENT 218 PURIFICATION SYSTEM EXTENDED PURIFICATION CAPABILITIES Agilent offers a range of purication modules with extended ow capabilities. For advanced sample handling, detection or fraction collection, Agilent has high performance modules to meet any purication challenge. Agilent 410 Autosampler 1 L to 5 mL injection volumes (up to 10 mL with preparative option) Pressure-assisted sample aspiration eliminates gas bubbles for excellent reproducibility Full-loop lling, partial- loop lling and L pick-up injection modes for highest exibility Agilent 325 Dual Wavelength UV-Visible Detector Detection in UV and visible light ranges from 190 to 900 nm Dual path-length ow cell for dynamic absorbance range 40 times greater than conventional detectors Simultaneously handling of two wavelengths for peak-purity assessment Agilent 440 Fraction Collector Real-time peak detection by Agilent OpenLAB CDS for accurate and reproducible fraction collection Fraction triggering by any combination of slope, threshold or time-slice Fast X, Z, theta-axis movement for minimal sample loss between collection vessels The dual path-length ow cell in the Agilent 325 Dual Wavelength UV-Visible Detector maximizes sensitivity for analytical applications while preventing detector overload during preparative runs. The Agilent 440 Fraction Collector is a random access, single probe fraction collector that can accommodate a variety of different sized vessels for full collection exibility. The Agilent 325 Dual Wavelength UV-Visible Detector has the unique capability to detect up to 80 AU on-scale without sacricing sensitivity for smaller peaks. For automated purication of multiple samples, the Agilent 410 Autosampler can be added to any Agilent purication solution. Achieve up to 80 AU with the 4 x 0.15 mm ow cell when moving from analytical to preparative operation, without changing the ow cell, as shown by the dynamic range from 2 to 21 AU for a 5-hydroxytryptophan sample. Learn more: www.agilent.com/chem/purication [mAU] [min] 1.0 1.5 2.0 2.5 3.0 3.5 10,000 5,000 15,000 20,000 6 AGILENT COLUMNS FOR LC PURIFICATION HIGHEST SAMPLE LOADING FOR RELIABLE PURIFICATION OF LARGE AMOUNTS AT LOWER COST Whether you are scaling up a routine analytical method, or maintaining precise separations throughout every phase of production, our wide array of preparative and process columns and bulk media are designed for high loadability in a range of particle sizes and phases. Semiprep and Prep Columns Agilent Prep LC columns are a cost-effective solution for high loadability to purify milligram to gram quantities with C18 and unbonded silica Agilent ZORBAX Prep HT columns are for rapid scale-up within the ZORBAX family, with optimized resolution and loadability under any conditions, up to 2,000 mg Agilent Pursuit and Pursuit XRs Prep columns offer high loadability with a high surface area, with C18, C8, Diphenyl, and Si, plus uorinated PFP and PAH polymeric for shape selectivity Agilent PLRP-S Prep columns span g/mg discovery to multiple-gram cGMP applications with a polymeric material that provides excellent chemical stability, up to 1 M NaOH, for column sanitation and regeneration Agilent PL-SAX and PL-SCX Prep columns have strong ion- exchange functionalities covalently linked to a chemically stable polymer for high-capacity purications, or large biomolecules with high-speed, high-resolution purications Bulk materials are available for most phases and can be ordered through Agilents Custom Ordering Process: www.agilent.com/chem/customlc Load & Lock Columns for Flexibility Available as 50 cm length column tubes with 1, 2 or 3 inch id Easy-to-use packing station allows you to pack any commercially-available media using dynamic axial compression (DAC) or static axial compression (SAC) Unique uid and sample distribution technology for increased sample loading, minimized peak broadening and reduced back pressure Agilent Prep LC columns provide the highest sample loading (by mass) in the industry so you can purify more sample in less time. A complete range of Agilent Load & Lock columns delivers versatile solutions for high performance, high throughput and high yield preparative and process purications. Proprietary uid and sample distribution plates at the inlet and outlet of the column diffuse the sample more efciently across the full bed surface, providing exceptional separation efciency, and a 20 % increase in load minimizes back pressure and peak broadening. Learn more: www.agilent.com/chem/lccolumns Agilent Load & Lock Columns Purify your way SD-1 Agilent SD-1 Purication System
Purify your way 7 AGILENT LC PURIFICATION SOLUTIONS PURIFY YOUR WAY WITH PURELY BETTER SOLUTIONS FOR COMPOUND ISOLATION Agilent offers the most comprehensive portfolio of exible and reliable solutions for purication by liquid chromatography. No matter what scale you are working at, Agilent has high-performance instrumentation, columns, software and services that ensure highest purity and maximum recovery. Download brochures Agilent Load & Lock Columns www.agilent.com/chem/LLbro 1260 Agilent 1260 Innity LC & LC/MS Purication Systems
Purify your way Agilent 1260 Innity LC & LC/MS Purication Systems www.agilent.com/chem/1260bro Agilent SD-1 Purication Systems www.agilent.com/chem/SD1bro Learn more: www.agilent.com/chem/purication *Optional software available for automated analytical-to-preparative scale-up Column Inside Diameter Productivity Range Flow Rate (mL/min) Agilent 1260 Innity Analytical Scale Agilent 1260 Innity Preparative Scale* Agilent 218 Agilent SD-1 4.6 mm inch (10 mm) 1 inch (2125 mm) (30 mm) 2 inch (50 mm) 3 inch (75 mm) 1 4.7 2025 42 118 265 Micrograms Grams Milligrams Analytical Semi-preparative Preparative Pilot 0.110 mL/min 20200 mL/min Flow range extensions made possible by exchangeable pump heads 500 mL/min 1100 mL/min 5100 mL/min 125 mL/min 1200 mL/min Learn more www.agilent.com/chem/purication Find an Agilent customer center www.agilent.com/chem/contactus USA and Canada 1-800-227-9770 [email protected] Europe [email protected] Asia Pacic [email protected] This information is subject to change without notice. Agilent Technologies, Inc. 2013-2014 Published in the USA, March 1, 2014 5991-1393EN