ABO Blood Group and Infertility

Research Article
Distribution of ABO blood group among fertile and

infertile males in central India: a pilot study
Bheem Prasad1, Adarsh Lalit2, Naresh C Sharma2
1
Department of Anatomy, All India Institute of Medical Sciences, Phulwari Sharif, Patna, Bihar, India.
Department of Biochemistry and Genetics,Barkatullah University, Bhopal, Madhya Pradesh, India.
Correspondence to: Bheem Prasad, E-mail: [email protected]
Received May 21, 2015. Accepted June 3, 2015
Abstract
Background: Blood is an important component of the human body that is responsible for complete human identity. The
ABO and Rh groups are very useful for blood transfusion, organ transplantation, biomedical research, and anthropological
study. The blood group incompatibility has been associated with some diseases.
Objective: To examine the relationship between blood group and infertility in men and to know the frequency of different
blood groups among infertile men who were referred from different infertility clinics and centers.
Materials and Methods: A total number of 88 infertile men along with 88 fertile men as controls were evaluated using the
antigenantibody agglutination test.
Result: The ABO blood group distribution among the infertile men was 40.90%, 29.54%, 19.31%, and 10.22% for blood
groups O, A, B,and AB, respectively, and the prevalence of ABO blood group in fertile were 44.31%, 27.27%, 22.72%, and
05.68% for groups O, A, B, and AB, respectively.
Conclusion: This preliminary study showed that the ABO blood group has no statistically significant association with male
infertility.
KEY WORDS: ABO blood group, antigenantibody, male infertility, reproduction, Rhesus factors
Introduction
Karl Landsteiner determined the ABO blood group system first time in 1901. There are about 400 blood grouping
antigens have been reported.[1] The ABO blood group and
Rhesus factors (Rh) are very useful in blood transfusion,
organ transplantation, genetic research, and anthropological
study in men.[2] Several studies demonstrated that the ABO
blood group is associated with some diseases. The ABO
blood group also more frequent in spontaneous miscarriage
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DOI: 10.5455/ijmsph.2015.21052015352
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or stillbirth.[3] Many studies have reported that the ABO blood

group is not related to infertility.[4,5] However, Khan et al.[6]
reported that the blood group O is related to male infertility.[6]
This study was undertaken to investigate any relationship
between ABO blood group and male infertility.
Materials and Methods

Study Design
A total number of 88 infertile men were enrolled during
July 2009 to January 2011 from different infertility clinics and
centers of Bhopal and Rewa district of Madhya Pradesh, India.
This study was approved by the ethical committee of the institute, and written consent from all the subjects was obtained.
In this, the population was divided into two groups:
Group A: 88 infertile men with mean age of 38.63 (7.13)
years were enrolled in this group, which revealed at least
2 years of infertility period. Hormone profile and semen analysis were also done.
International Journal of Medical Science and Public Health Online 2015. 2015 Bheem Prasad. This is an Open Access article distributed under the terms of the Creative
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International Journal of Medical Science and Public Health | 2015 | Vol 4 | Issue 12 (Online First)
Prasad et al.: ABO blood group among fertile and infertile male subjects
Group B: The control group comprised 88 fertile men

with mean age 33.57 (5.96) years who had at least two live
issues without any abnormalities.
Methodology
Slide agglutination method was used for confirming blood
group of all men, after obtaining their informed consent. Two
milliliters of whole blood was taken from each subject by
antecubital vein puncture with aseptic conditions for antigen
antibody agglutination test. A few drops of blood were kept
on clean slides. A drop of each of the antisera was added
and mixed with each blood drop. ABO blood groups were
confirmed by antigenantibody agglutination test.[7]
Results
A total of 88 infertile men were included in this study.
Data were recorded for their age, height, and weight. The
average ages of infertile and fertile men were 38.63 (7.13) and
33.57 (5.96) years,respectively. The maximum infertile male
population was found between the age group of 3040 years,
comprising approximately 50% of the entire population.
The ratio of patients to control was 1:1. The distribution
of ABO blood group in infertile men shown the maximum
frequency of O blood group, followed by blood groups A and B,
while the AB blood group was less common in both infertile
and fertile men.
The distribution of blood groups in infertile and fertile
men is shown in Table 1. The prevalence of infertile men with
blood groups O, A, B, and AB was 36 (40.90%), 26 (29.54%),
17 (19.31%), and 9 (10.22%), respectively, as shown in Table 1.
The distribution of ABO blood group in fertile men showed
that 39 (44.31%), 24 (27.27%), 20 (22.72%), and 5 (5.68%)
were for groups O, A, B, and AB, respectively. There was
no statistically significant difference between the distribution
of the infertile and fertile men as shown in Table 1 (2 = 1.59,
P = 0.66).
Discussion
The frequency distribution of ABO blood group in the
infertile group was the approximately same with fertile group.
There are no any significant differences in ABO blood group
distribution among both the infertile and fertile men. Distri

bution of blood groups in infertile men showed the maximum
frequency of O blood group, followed by blood groups A, B,
and AB.
Similar studies conducted by Cantuaria,[8] Schwimmer
et al.,[9] and Omu et al.[10] showed no significant relation
between ABO blood group antigens and infertility. Solish and
Gershowitz[4] reported that there is no any relation between
blood groups in husbands and wives of infertile and fertile
group.[4] Similarly, Lurie et al.[11] demonstrated that the association of the Lewis blood group with infertility in among groups
was not statistically different, but Khan et al.[6] found strong
relation between blood group O and male infertility.[6,11]
Seminal blood group antigens were found on the surface of
spermatozoa, which may affect fertility in humans.
Many studies emphasized the correlation of ABO blood
group with some diseases. Aird et al.[12] reported that carcinoma of the stomach is related to blood group A, whereas
Shamim et al.[13] reported that a blood group is directly associated with cardiovascular diseases, atherosclerotic peripheral
vascular diseases, and other type of cardiovascular disease.
Singh et al.[14] showed that malaria infection is found more in
blood groups A, B, and O when compared with blood group AB.
It is interesting to note that blood group A pose a significant higher risk for gastric cancer and smallpox, and blood
group B is associated with ovarian, cervix, lung, and buccal cancers. Moreover, blood group AB is associated with
ischemic heart disease and venous thromboembolism.
Blood group O is associated with breast cancer, cholera,
and gastrointestinal (GI) infection by Escherichia coli and
GI ulceration.[1517] Hadeal and Ali[18] showed that blood groups
B and O+ are associated with diabetes mellitus and hypertension.
Behrman et al.[19] reported that 30%40% of infertile
couples revealed ABO incompatibility. Allan[20] found that there
are no significant correlation between blood group ABO and
human infertility. However, Ogbimi et al.[21] reported that ABO
compatibility and incompatibility did not reveal any statistically
significant difference.
Conclusion
There was no significant correlation between the ABO
blood group and infertility in male subjects. The prevalence of
Table 1: Comparison between the ABO blood group of infertile male (group A) and fertile male (group B) subjects
Blood
group
A
B
AB
O
Group A (infertile men),

n (%)
Group B (fertile men),

n (%)
Total (groups A+ B), n
Significance
17 (19.31)
24 (27.27)
20 (22.72)
50
0.06
0.81
Not significant
05 (05.68)
37
14
0.60
0.44
Not significant
26 (29.54)
09 (10.22)
36 (40.90)
39 (44.31)
75
0.38
0.04
0.54
0.84
Not significant
Not significant
Prasad et al.: ABO blood group among fertile and infertile male subjects
blood group O is higher in infertile and fertile groups followed

by blood groups A and B, whereas, the least prevalence was
blood group AB. Large-scale studies are required to verify
this correlation for appropriate intervention strategies. This
observation could contribute to the field of andrology and for
clinicians who are dealing infertility cases in men.
Acknowledgment
We acknowledge Dr.Sarvesh Saxena, Saxena Infertility
and Diagnostic Research Center, Rewa, and Dr. Abha Jain,
Life Line Hospital, Bhopal, for providing the samples for the
study.
References
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How to cite this article: Prasad B, Lalit A, Sharma NC.
Distribution of ABO blood group among fertile and infertile males
in central India: a pilot study. Int J Med Sci Public Health 2015;4
(Online First). DOI: 10.5455/ijmsph.2015.21052015352
Source of Support: Nil, Conflict of Interest: None declared.

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ABO Blood Group and Infertility

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Research Article

Distribution of ABO blood group among fertile and

Received May 21, 2015. Accepted June 3, 2015

Quick Response Code:

or stillbirth.[3] Many studies have reported that the ABO blood

Materials and Methods

Group B: The control group comprised 88 fertile men

distribution among both the infertile and fertile men. Distri

Group A (infertile men),

Group B (fertile men),

Total (groups A+ B), n

blood group O is higher in infertile and fertile groups followed

Source of Support: Nil, Conflict of Interest: None declared.

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