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    Transmission Electron Microscope (TEM)

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    Chandrashekhar Singh
    Principle, Diagram, Working, Construction, Uses & Application of Transmission Electron Microscope in field of pharmacy.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Transmission Electron Microscope (TEM)

    Uploaded by

    Chandrashekhar Singh
    586 views10 pages
    Principle, Diagram, Working, Construction, Uses & Application of Transmission Electron Microscope in field of pharmacy.

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    © © All Rights Reserved

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    PDF, TXT or read online from Scribd

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    Principle, Diagram, Working, Construction, Uses & Application of Transmission Electron Microscope in field of pharmacy.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    586 views10 pages

    Transmission Electron Microscope (TEM)

    Uploaded by

    Chandrashekhar Singh
    Principle, Diagram, Working, Construction, Uses & Application of Transmission Electron Microscope in field of pharmacy.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 10

    Transmission Electron

    Microscope (TEM)
    Prepared bySiddhi Rakshe
    Chandrashekhar Singh
    Ashwin Kewat

    Principle
    The Source of illumination in TEM is electron beam. Resolution of
    microscope depends on wavelength of light.
    Use of light with low wavelength is responsible for increasing
    resolution
    The Electron Beam used in this microscope in this microscope has
    wavelength of 5nm (Compared to wavelength of light rays -280nm to
    8oonm)
    Electrons have low penetration power, so a very thin film of specimen
    can be observed
    The magnification can be achieved upto 105

    Construction
    Transmission Electron
    Microscope consists:

    Power source
    Electron gun
    Central column (Microscope Column)
    Electromagnetic lens
    Fluorescence screen.
    Vacuum pump

    Working:
    A high-voltage electricity supply powers the cathode.
    The cathode is heated filament, a bit like the electron gun in an oldfashioned cathode-ray tube (CRT) TV. It generated a beam of
    electrons that works In an analogous way to the beam of light in an
    optical microscope.
    An electromagnetic coil (The first lens) concentrates the electon into a
    more powerful beam
    Another electromagnetic coil focuses the beam onto a certain part of
    the specimen.
    The specimens sits on a copper grid in the middle of main microscope
    tube. The beam passes through the specimen and picks up an
    image of it.

    Working (Cont.)
    The projector lens (the third lens) magnifies the
    image.
    The image becomes visible when the electron beam
    hits a fluorescent screen at the base of the machine.
    This is analogous to the phosphorscreen at the front
    of an old-fashioned TV.
    The image can be viewed directly (through a viewing
    portal), through binoculars at the side, or on a TV
    monitor attatched to an image intensifier

    Appication of TEM
    TEMs are capable of imaging at a significantly
    higher resolution than light microscopes, owing to the small
    wavelength of electrons. This enables the instrument's user
    to examine fine
    TEM forms a major analysis method in a range of scientific
    fields, in physical, chemical and biological sciences. TEMs
    find application in cancer research, virology, materials
    science as well as pollution,nanotechnology,
    and semiconductor research.

    Advantages of TEM
    TEMs offer the most powerful magnification, potentially over one
    million times or more
    TEMs provide information on element and compound structure
    Images are high-quality and detailed
    TEMs are able to yield information of surface features, shape, size
    and structure in 3D

    Disadvantages of TEM
    Images are black and white
    Electron microscopes are sensitive to vibration and electromagnetic
    fields and must be housed in an area that isolates them from possible
    exposure
    TEMs are large and very expensive
    Samples are limited to those that are electron transparent, able to
    tolerate the vacuum chamber and small enough to fit in the chamber

    Thank you

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