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Integrative Oncology
ISSN: 2329-6771
Research Article
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Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India
Abstract
Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The
aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two
time-lapse video microscopy technique.
Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM
treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. nonGBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedis biofield
treatment for the assessment of its potential. Two time-lapse (10 hours prior; 10 hours after) video microscopy
experiment was performed on tumor and non-tumor brain cells in six replicate (n=6). For each microscopic field, the
total cell number was counted and each cell was tracked over the 20 hours period. The potential impact of biofield
treatment was assessed by comparing cell death rate in both GBM and non-GBM cells before and after biofield
treatment.
Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield
treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death
rate was exponentially increased (41%) after biofield treatment as compared to control. The treated non-GBM
cultured cells showed a significant reduction (64%) of cell death rate i.e. protective effects as compared to non-GBM
control.
Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of
treated GBM cells and simultaneously boost the viability of normal brain cells. Therefore, biofield treatment could be
a suitable alternate treatment strategy for cancer patients in near future.
Introduction
Brain tumors are typically very heterogeneous, aggressive
neoplasms at the cellular level which affects both children and adults
[1]. Statistics based on cancer research institute explore that 1 in 161
individuals born today with brain cancer at different point of life. In the
U.S., 22,850 men and women are diagnosed with brain cancer every year,
and 15,320 deaths are caused by this disease [2]. As per World health
organization classification, glioblastoma (GBM) is also known as grade
IV astrocytoma [3]. GBM is one of the most malignant form of human
brain tumor. The mean survival time for GBM patients is approximately
12 months [4]. Time-lapse video microscopy is a technique that aids to
assess cellular behavior in real time. This technique is used to collect
a two dimensional image data at different time intervals. Then, these
data are converted to make a movie. This unique technique is used
in various fields of cancer and stem cell biology for assessment of
cell translation, division, and death [5,6]. Presently, several strategies
are available for the treatment of GBM such as surgical techniques,
radiotherapy, chemotherapy and electrochemotherapy [7]. Apart
from commonly used chemotherapeutic agents (such as nitrosoureas
derivatives, platinum based drugs, and taxol) and gene therapy, as
a novel therapeutic modality frequently being used in cancer [8].
However, chemotherapy suffers several major drawbacks such as all
tumors are not responsive to chemotherapeutic agents to some extent,
incidence of bone marrow cells damage, amenorrhea, alopecia, sexual
dysfunction and adversely affect the quality of life of patients [9].
Malignant brain tumors like GBM are very difficult to treat
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal
now a days. The difficulties to treat against GBM are due to lack of
proper preventive strategies, and unavailability of practical method
for screening [10]. Therefore, new, more effective and better tolerated
anti-tumor drugs or some alternative treatment strategy are needed.
Based on the above lacunas an alternative way which may be useful to
kill or eradicates the proliferative tumorous cells and simultaneously
defense the normal brain glial cells. Biofield treatment is an alternative
approach which may be useful to improve these unfilled spaces
associated with cancer patients. The human biofield is the energetic
matrix that surrounds the human [11]. It directly links with the cellular
activity that allows the DNA to communicate faster than light and
maintain intelligence in the organisms [12]. According to universal
principles of Maxwells equations and principle of reciprocity defines
electromagnetic connections related to human biofield [13]. Thus, a
human has ability to harness the energy from environment/Universe
and can transmit into any object (living or non-living) around the
Globe. The object(s) always receive the energy and responded into
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A TimeLapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 2 of 4
useful way that is called biofield energy. This process is known as biofield
treatment. The biofield can be monitored using electromyography,
electrocardiography and electroencephalogram [14]. Mr. Mahendra
Trivedis unique biofield treatment (The Trivedi Effect) has been
well known for altered characteristics features of microbes [15-17],
improved the overall productivity of crops [18,19], and also transform
the structural, physical and chemical properties of materials [20-23].
Exposure to biofield energy caused an increase in medicinal property,
growth, and anatomical characteristics of ashwagandha [24].
On the basis of above facts and literature, present study was
undertaken to evaluate the impact of Mr. Trivedis biofield treatment
on human GBM brain tumor cells.
the microscopic field after the initial frame were neither included,
nor identified as dead at the start of the video. Cell death, divisions
and emigrations (cells migrating out of the microscopic field) were
counted for varying numbers of cells and count was made every single
hour. After that, all the data were collected and entered into excel
spreadsheets, the blinding codes were revealed and the results were
sorted according to experimental group for analysis.
Where, N(t-1) is the number of cells at the start of the time period
and d(t) is the number of deaths during the time period. These estimates
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A TimeLapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 3 of 4
are based on an assumption that counts are dependent on the number
of cells present [6].
Comparing the total number of cell deaths over the 10 hours prior
and 10 hours following the treatment time (T = 0) for the four groups of
cells and dividing these values by the initial cell counts for each group
gives a rough estimation of the potential effect of treatment. Analysis
using the exponential growth model indicates that the cell death rate
in GBM treatment cultured cells increased after biofield treatment
however, no change in cell death rate was observed in GBM control
cultured cells. Moreover, the non-GBM treatment cultured cells has
appeared to be protected from a baseline increase in cell death rate
observed in the untreated non-GBM control cultured cells (Figure 3).
The increase in cell death rate as observed in the untreated non-GBM
control cultured cells was consistent while the cancer cell viability was
more robust [29].
Pre-treatment values were subtracted from post-treatment values
to evaluate the relative change in cell death rate after treatment time (T
= 0) and referred as delta (). The values (as measured from before
to after treatment per initial cell) due to biofield treatment relative
to controls for GBM cells and non-GBM cells are shown in Figure
4. The advantage of biofield treatment is that they are inexpensive as
compared to other types of cancer therapies. According to a recent
report regarding biofield treatment later on, it was approved by the
German equivalent of the FDA. It was emphasized that cancer patients
have experienced healing with biofield treatment. Nowadays, many
scientists and cutting edge practitioners believed that the secrets
of regeneration and healing lie not only on costly medical drugs or
expensive medical treatments, but also in the bodys own Quantum
Energy Biofield (QEB) [30].
Current cancer therapies have certain limitations which include
serious side effects like enormous toxicity, altered immune system and
high treatment cost. Many ancient biofield therapies for cancer are
practiced around the world, among then Qigong therapy for cancer is
Before
0.8
After
0.7
0.6
0.5
0.4
0.3
GBM
Non-GBM
-0.1
-0.2
-0.3
-0.4
-0.5
Figure 4: The change in cell death rate () of both glioblastoma brain tumor
cells and normal brain glial cells after treatment time (T = 0) due to biofield
treatment relative to controls.
Figure 5: Percent change of cell death after 20 hours treatment period with
respect to control.
Conclusions
0.2
0.1
0
0.1
0.2
Control
Treatment
GBM
Control
Treatment
Non GBM
Figure 3: Comparison of cell death rate before and after biofield treatment.
GBM: Glioblastoma
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A TimeLapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 4 of 4
biofield treatment approach could be used in glioblastoma brain tumor
patients in near future to improve the quality of life.
Acknowledgement
Trivedi science, Trivedi testimonials and Trivedi master wellness gratefully
acknowledged to the whole team of the bioscience laboratories, 665, Third Street,
San Francisco, CA for their support.
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Special features:
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The
Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A
Time-Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/23296771.1000141
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal